FastQCFastQC Report
Mon 6 Jun 2016
ERR1211632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412072
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163890.3714581267032814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135620.307383923018482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91490.20736288981684795No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83760.18984277681778539No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47290.10718320099943972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58500.050.4995232
GTACCTG60200.049.519231
ACCTGGG70850.041.114353
GGTATCA92100.040.2546651
TATAACG5400.039.2014582
TAACGCA5550.038.9413834
ATAACGC6000.035.237323
GTATCAA141000.032.8842351
CCTGGGG64350.028.9134
GTACATG137750.028.900421
TCAACGC167750.027.2799114
ATCAACG168650.027.0504473
CAACGCA170250.026.9323795
TACATGG143000.026.9091992
AACGCAG177050.026.1070066
ACATGGG142300.026.0173423
TATCAAC175950.026.0139962
CATGGGG89600.024.592994
ACGCAGA196700.023.3081767
CGCAGAG200400.022.690578
Produced by FastQC (version 0.11.5)