FastQCFastQC Report
Mon 6 Jun 2016
ERR1211632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412072
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183820.416629647022986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160340.36341202047473387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116530.26411627008806743No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85590.1939904879158817No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49280.11169355350501987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58950.053.048052
GTACCTG60550.052.401911
ACCTGGG67350.045.3505333
TATAACG5100.044.259112
GGTATCA105400.037.75271
TAACGCA6550.036.586494
CCTGGGG60250.032.755494
GTATCAA161850.032.4794271
ATAACGC7800.030.1218783
GTACATG147550.027.9585761
TACATGG150550.026.5815562
TCAACGC198900.026.0338674
ATCAACG200000.025.8680673
CAACGCA200700.025.8237935
ACATGGG148150.025.6598133
TATCAAC205700.025.3758342
AACGCAG208750.024.963866
CATGGGG88850.023.63974
GTATAAC14600.022.6043171
ACGCAGA234850.022.1294787
Produced by FastQC (version 0.11.5)