FastQCFastQC Report
Mon 6 Jun 2016
ERR1211631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5633108
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194000.344392473923809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159270.2827391202156962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109930.1951498178270326No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76860.13644332755558744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90350.047.544261
GTACCTG72800.046.893761
TACCTGG71500.046.7765122
ACCTGGG82150.039.921843
GTATCAA153250.036.706911
GTACATG190150.028.8397331
TCAACGC188950.028.7706184
TATAACG6550.028.7265172
ATCAACG190650.028.489433
CAACGCA191150.028.3880585
AACGCAG198350.027.4733356
TAACGCA6400.027.163424
TACATGG197150.027.1524942
TATCAAC200450.027.1278932
ACATGGG197300.026.1003423
CCTGGGG82700.025.3391514
CATGGGG126450.024.3380154
ACGCAGA224150.024.1227057
ATAACGC7600.024.1109183
CGCAGAG231800.023.0854478
Produced by FastQC (version 0.11.5)