FastQCFastQC Report
Mon 6 Jun 2016
ERR1211631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5633108
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230780.4096850264543126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193500.3435048644549332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141500.25119347969185035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83030.14739642840151476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72200.050.276792
GGTATCA104200.048.8111231
GTACCTG75400.048.6377331
ACCTGGG81550.043.5016563
TATAACG7600.037.1214682
GTATCAA177400.036.030571
TAACGCA7600.035.8586044
ATAACGC8900.033.2608533
CCTGGGG82100.029.53154
TCAACGC222900.028.099534
ATCAACG225450.027.8236333
CAACGCA227400.027.605465
TATCAAC234300.026.9319422
AACGCAG238200.026.611686
GTACATG198000.026.330251
TACATGG204250.024.9778542
ACATGGG201500.024.2053
ACGCAGA270050.023.3686667
CATGGGG129400.023.094144
CGCAGAG277550.022.5509648
Produced by FastQC (version 0.11.5)