FastQCFastQC Report
Mon 6 Jun 2016
ERR1211630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466413
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304900.3220860953351602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268580.2837188700725396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174300.18412465207254322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156750.049.5238721
GTATCAA276150.036.6331751
GTACATG352200.030.8923231
TCAACGC321600.030.4326824
CAACGCA324550.030.138725
ATCAACG326050.029.9612713
TACATGG367450.029.1929262
AACGCAG337550.029.0862676
TACCTGG78600.028.7315922
ACATGGG364100.028.54663
GTACCTG83250.027.8723221
TATCAAC354450.027.8212932
CATGGGG198050.025.5035484
ACGCAGA384450.025.2938487
CGCAGAG392550.024.5457178
GCAGAGT426950.021.7645539
ACCTGGG101450.021.7225463
CATGGGA157250.020.7962764
GAGTACA249850.020.763741
GAGTACT267250.020.43801912-13
Produced by FastQC (version 0.11.5)