FastQCFastQC Report
Mon 6 Jun 2016
ERR1211630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466413
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364840.38540469341449607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325390.3437310415254437No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226310.23906626512069568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172900.047.1651041
GTATCAA299150.037.045321
TCAACGC364350.029.5451344
CAACGCA367700.029.2374655
ATCAACG368650.029.2261583
TACCTGG73050.028.8378772
GTACATG372600.028.325721
AACGCAG381400.028.2988726
TATCAAC394150.027.4869422
GTACCTG79250.027.182331
TACATGG391950.026.7054562
ACATGGG380100.026.4420343
CATGGGG206050.024.7193094
ACGCAGA437050.024.5662737
CGCAGAG447650.023.900598
ACCTGGG96050.021.5247043
GCAGAGT492450.021.4872729
ATGGGGG115650.020.964355
GAGTACT301450.019.92774812-13
AGAGTAC459250.019.7410710-11
Produced by FastQC (version 0.11.5)