FastQCFastQC Report
Mon 6 Jun 2016
ERR1211629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6795615
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189440.2787679996586034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160650.23640244481183825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107770.15858755977200004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102750.045.4994851
GTATCAA169850.036.3015141
GTACCTG64400.030.8397561
TACCTGG62700.030.8394322
TCAACGC199200.030.1691534
CAACGCA199950.029.9360715
ATCAACG201150.029.8306263
GTACATG210300.029.2518271
AACGCAG208450.028.688816
TATCAAC214100.028.2369982
TACATGG217750.027.8501032
ACATGGG219750.026.6214683
ACGCAGA239250.024.7206467
CGCAGAG243950.024.2260078
ACCTGGG78350.023.989023
CATGGGG128900.023.5484054
TATAACG5550.022.8877372
GCAGAGT272500.021.1530829
ATGGGGG77850.020.2781095
GAGTACT174200.019.56796312-13
Produced by FastQC (version 0.11.5)