FastQCFastQC Report
Mon 6 Jun 2016
ERR1211629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6795615
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227450.334701127123888No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198510.29211484170306884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140200.2063095098824757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110450.045.843711
GTATCAA188450.037.6764561
TACCTGG63550.032.8554272
GTACCTG66400.031.7380811
TCAACGC230100.030.2033844
ATCAACG232100.029.9233173
CAACGCA232500.029.8509655
AACGCAG242400.028.6520356
TATCAAC245100.028.4919952
GTACATG218100.026.5883061
ACCTGGG78600.025.7670783
TATAACG7500.025.7076452
TACATGG226500.025.2882522
ACATGGG223850.025.0224383
ACGCAGA275950.024.9804677
TAACGCA8300.024.344144
CGCAGAG282900.024.2338948
CATGGGG129200.024.0768414
GCAGAGT319650.021.1094749
ATGGGGG78900.020.546745
Produced by FastQC (version 0.11.5)