FastQCFastQC Report
Mon 6 Jun 2016
ERR1211628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7717967
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229190.2969564394354109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198600.25732164960021203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130430.1689952807520426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129650.046.792851
GTATCAA223450.036.9251
TCAACGC263300.030.2518444
CAACGCA266700.029.862315
GTACATG284600.029.8199771
ATCAACG268050.029.6970793
AACGCAG276350.028.9331156
TACATGG291900.028.5952132
TATCAAC282850.028.3623352
ACATGGG291450.027.9254363
CATGGGG165950.026.2503744
ACGCAGA311500.025.4571347
GTACCTG64350.025.3652171
TACCTGG63350.024.8069672
CGCAGAG319500.024.7473148
GCAGAGT358300.021.477419
TAACGCA6250.021.0528564
ATGGGGG97750.020.6213595
GAGTACT215200.020.27305412-13
AGAGTAC324150.019.74968510-11
Produced by FastQC (version 0.11.5)