FastQCFastQC Report
Mon 6 Jun 2016
ERR1211628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7717967
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262820.3405300903722444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235250.3048082480788011No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162680.21078089605721298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144900.047.520821
GTATCAA238550.035.7650341
TCAACGC292950.028.423474
CAACGCA294800.028.2614085
ATCAACG295800.028.1337283
GTACATG281750.027.6029951
TAACGCA7550.027.3849934
AACGCAG305550.027.2833636
TATCAAC311900.026.872982
GTACCTG67400.026.5902981
TACCTGG65650.026.2938882
TACATGG290650.026.2322882
TATAACG7600.025.9931682
ACATGGG288450.025.2340973
ACGCAGA347050.023.830967
CGCAGAG356900.023.0547588
CATGGGG164000.022.9220264
ACCTGGG81100.020.5690573
GCAGAGT394700.020.4063199
ATGGGGG92200.020.1316185
Produced by FastQC (version 0.11.5)