FastQCFastQC Report
Mon 6 Jun 2016
ERR1211627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789203
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240840.2460261575942393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209210.21371504912095501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136840.13978666087525207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134950.045.718061
GTATCAA232550.036.0635381
TCAACGC270000.029.6899494
ATCAACG271550.029.5541823
CAACGCA272950.029.4331155
GTACATG285550.028.9899791
AACGCAG284100.028.4234496
TATCAAC294450.027.5640142
TACATGG295450.027.279622
ACATGGG296450.026.247613
ACGCAGA322500.024.7335687
CGCAGAG332050.023.754878
CATGGGG184550.023.1188354
GCAGAGT373750.020.5513239
GAGTACT231900.019.74380712-13
ATGGGGG110550.019.3789065
GTACCTG82100.019.131721
AGAGTAC336350.019.07387710-11
TACCTGG78800.018.3903262
CAGAGTA367550.018.10668210-11
Produced by FastQC (version 0.11.5)