FastQCFastQC Report
Mon 6 Jun 2016
ERR1211627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789203
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288280.2944877126360543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252620.25805982366490915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172670.17638821056218773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149700.045.8902631
GTATCAA253650.036.6382371
TCAACGC310300.029.1816254
ATCAACG312900.028.9699213
CAACGCA313000.028.9294575
AACGCAG324950.027.9388796
TATCAAC332900.027.4201432
GTACATG295950.025.9847411
TACATGG299250.025.1445452
ACGCAGA368950.024.466617
ACATGGG302000.024.0248663
CGCAGAG379650.023.6532758
GTACCTG76200.022.2137681
CATGGGG186250.021.6976014
TACCTGG76600.021.3038772
GCAGAGT423700.020.783389
AGAGTAC386750.019.70115710-11
GAGTACT271300.019.66699612-13
ATGGGGG106150.019.5218735
AGTACTT288150.018.32940512-13
Produced by FastQC (version 0.11.5)