FastQCFastQC Report
Mon 6 Jun 2016
ERR1211626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6521592
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192360.2949586542672403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165850.25430907054596485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110380.16925315168443533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101250.044.3499951
GTATCAA169550.037.640181
TACCTGG61000.034.0904052
GTACCTG65300.033.0829281
TCAACGC201150.030.627094
ATCAACG202550.030.4381373
CAACGCA203900.030.3260025
TATCAAC214050.029.1892242
AACGCAG213000.029.1584936
GTACATG205450.027.4125751
ACCTGGG76150.026.4113313
TACATGG211350.026.1785012
ACGCAGA241250.025.374477
ACATGGG214650.025.0225623
CGCAGAG248450.024.4695038
CATGGGG135250.024.043184
TATAACG6550.022.2669182
TAACGCA6700.021.0411554
GCAGAGT279600.021.0206769
GAGTACT174250.019.92667412-13
Produced by FastQC (version 0.11.5)