FastQCFastQC Report
Mon 6 Jun 2016
ERR1211626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6521592
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225950.346464482905401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201800.309433647489754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138590.21250946088010414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115050.045.645721
TACCTGG65200.037.5082052
GTACCTG69100.036.0893551
GTATCAA186700.035.299161
ACCTGGG78950.030.3551313
TCAACGC233950.027.437114
ATCAACG235550.027.3107993
CAACGCA237200.027.1596075
GTACATG213500.026.452121
TATCAAC246950.026.2999652
AACGCAG245950.026.2128776
TACATGG217150.025.534332
TATAACG6650.025.4595872
ACATGGG217300.024.47943
ACGCAGA280700.022.7834667
CATGGGG129850.022.5453434
CGCAGAG291250.021.8775088
TAACGCA6950.020.28374
GCAGAGT323550.019.4609559
AGAGTAC293250.019.19653510-11
Produced by FastQC (version 0.11.5)