FastQCFastQC Report
Mon 6 Jun 2016
ERR1211625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6345306
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191090.30115174902518493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164700.25956195020381995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109230.1721429983045735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91150.048.938261
GTATCAA159300.038.0957761
TACCTGG52000.036.996742
GTACCTG54050.036.2048261
TCAACGC191650.030.7184524
ACCTGGG60850.030.5767753
GTACATG197200.030.5110971
CAACGCA193100.030.460095
ATCAACG194450.030.1794683
AACGCAG201500.029.3038626
TATCAAC204800.028.9620042
TACATGG212150.027.869922
ACATGGG211300.027.0612683
ACGCAGA230800.025.1567297
CGCAGAG233300.024.7067178
ATGGGGG72150.024.0920935
CATGGGG151350.022.879274
GCAGAGT256550.021.845089
TATAACG4150.021.5352042
CCTGGGG62900.020.0936454
Produced by FastQC (version 0.11.5)