FastQCFastQC Report
Mon 6 Jun 2016
ERR1211625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6345306
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232460.36634955036053424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204260.32190724923273994No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143630.22635630180798216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105800.048.2850761
TACCTGG54850.038.66362
GTATCAA179950.036.7430421
GTACCTG58000.036.7265171
ACCTGGG64800.032.4138223
TCAACGC225250.028.995734
ATCAACG226400.028.870113
CAACGCA226450.028.8423025
GTACATG206050.028.0634611
TATCAAC236700.027.7524012
AACGCAG236350.027.71486
TATAACG5150.026.4784892
TACATGG220050.025.7921962
ACATGGG216500.025.4153863
ACGCAGA270000.024.1561017
CGCAGAG276050.023.473498
ATGGGGG70000.023.1583985
CATGGGG147600.023.1118284
TAACGCA5750.022.8809594
ATAACGC6800.022.8035663
Produced by FastQC (version 0.11.5)