##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6345306 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01308352977776 33.0 31.0 34.0 30.0 34.0 2 32.28134119930544 34.0 31.0 34.0 30.0 34.0 3 32.37265421084499 34.0 31.0 34.0 30.0 34.0 4 36.013612424680545 37.0 35.0 37.0 35.0 37.0 5 35.90592037641684 37.0 35.0 37.0 35.0 37.0 6 35.94108826272523 37.0 35.0 37.0 35.0 37.0 7 35.84580176275187 37.0 35.0 37.0 35.0 37.0 8 35.832115109972634 37.0 35.0 37.0 35.0 37.0 9 37.456750076355654 39.0 37.0 39.0 34.0 39.0 10-11 37.42507942091366 39.0 37.0 39.0 34.0 39.0 12-13 37.33063472431432 39.0 37.0 39.0 34.0 39.0 14-15 38.55638947908895 40.0 38.0 41.0 34.0 41.0 16-17 38.487242065236885 40.0 38.0 41.0 34.0 41.0 18-19 38.48087239606727 40.0 38.0 41.0 34.0 41.0 20-21 38.41392605809712 40.0 38.0 41.0 34.0 41.0 22-23 38.316039919903 40.0 38.0 41.0 34.0 41.0 24-25 38.21296419747132 40.0 38.0 41.0 33.5 41.0 26-27 38.00370959257127 40.0 37.0 41.0 33.0 41.0 28-29 38.02510052312686 40.0 37.0 41.0 33.0 41.0 30-31 37.90958694505828 40.0 37.0 41.0 33.0 41.0 32-33 37.73747586010825 40.0 37.0 41.0 33.0 41.0 34-35 37.60957178424492 40.0 37.0 41.0 32.5 41.0 36-37 37.52972725034853 40.0 37.0 41.0 32.0 41.0 38-39 37.466900571855795 40.0 37.0 41.0 32.0 41.0 40-41 37.31549558051259 40.0 36.0 41.0 31.0 41.0 42-43 37.14529275656682 39.5 36.0 41.0 31.0 41.0 44-45 36.9619975774218 39.0 36.0 41.0 30.5 41.0 46-47 36.72791446149327 39.0 35.5 41.0 30.0 41.0 48-49 36.49775140867911 39.0 35.0 40.5 30.0 41.0 50-51 36.31196935183268 39.0 35.0 40.0 29.0 41.0 52-53 36.110801039382494 39.0 35.0 40.0 28.0 41.0 54-55 35.90616071155591 38.5 35.0 40.0 28.0 41.0 56-57 35.70434704015851 38.0 34.0 40.0 27.5 41.0 58-59 35.44539262566691 38.0 34.0 40.0 27.0 41.0 60-61 35.14185943120789 38.0 34.0 40.0 26.0 41.0 62-63 34.813303724044204 37.5 33.5 40.0 26.0 41.0 64-65 34.498657590351044 37.0 33.0 40.0 26.0 41.0 66-67 34.13218093816121 36.0 32.5 39.0 25.0 41.0 68-69 33.748931336014365 36.0 32.0 39.0 24.5 40.0 70-71 33.36488586996435 35.5 32.0 38.5 23.5 40.0 72-73 32.93336475813775 35.0 31.0 38.0 22.5 40.0 74-75 32.52677514685659 35.0 31.0 37.0 22.0 39.0 76-77 31.033252218253935 33.5 29.5 35.5 20.5 38.5 78-79 31.87009483860983 34.0 30.5 36.0 22.0 39.0 80-81 31.809729349538067 34.0 31.0 36.0 22.0 37.5 82-83 31.50409956588382 34.0 30.5 35.5 21.0 37.0 84-85 31.161879899881896 34.0 30.0 35.0 20.0 37.0 86-87 30.84473664469452 34.0 30.0 35.0 20.0 36.0 88-89 30.55035777628376 34.0 29.0 35.0 19.5 36.0 90-91 30.268027972173446 34.0 29.0 35.0 18.5 35.5 92-93 30.031770965813152 34.0 29.0 35.0 18.0 35.0 94-95 29.8148721275223 34.0 29.0 35.0 18.0 35.0 96-97 29.578337120384738 33.5 29.0 35.0 17.0 35.0 98-99 29.361189357928524 33.0 27.5 35.0 16.5 35.0 100 27.5329695683707 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20869708134810594 1101 2 0.14256293983364543 1101 3 0.12461871963960647 1101 4 0.07750367938663061 1101 5 0.07360279355411592 1101 6 0.0989344759182984 1101 7 0.07017517758804104 1101 8 0.07678929932823308 1101 9 0.08306898086544123 1101 10-11 0.06860209654514193 1101 12-13 0.015240655172526374 1101 14-15 0.03977027576743808 1101 16-17 0.09011171360727843 1101 18-19 0.07883764362070167 1101 20-21 -0.017765964267567824 1101 22-23 -0.04790609386233058 1101 24-25 -0.07026746254798155 1101 26-27 -0.03941540101820351 1101 28-29 -0.009905373976152987 1101 30-31 0.0377313127986838 1101 32-33 -0.007162885095489457 1101 34-35 0.04008770305330245 1101 36-37 -0.03826818915189989 1101 38-39 -0.16592610283812803 1101 40-41 -0.12662003637412766 1101 42-43 -0.07651951262739232 1101 44-45 -0.12584594345783273 1101 46-47 -0.237628834475764 1101 48-49 -0.1541664642315297 1101 50-51 -0.2805096331209995 1101 52-53 -0.29527482551679185 1101 54-55 -0.3083254440237724 1101 56-57 -0.30304660128933136 1101 58-59 -0.38013744849485676 1101 60-61 -0.4211030085934766 1101 62-63 -0.526652426737094 1101 64-65 -0.5459679924867729 1101 66-67 -0.5567473364403313 1101 68-69 -0.5545071764582303 1101 70-71 -0.6020283832231286 1101 72-73 -0.6885224865545396 1101 74-75 -0.697045244148903 1101 76-77 -0.6469026976860945 1101 78-79 -0.6015666649751239 1101 80-81 -0.5755032701284293 1101 82-83 -0.6741109764406765 1101 84-85 -0.7642604394326113 1101 86-87 -0.8217259145769766 1101 88-89 -0.8004671097809073 1101 90-91 -0.9130708941036723 1101 92-93 -1.0198810805225023 1101 94-95 -1.0403818316562372 1101 96-97 -1.1714498299949518 1101 98-99 -1.165193432698775 1101 100 -1.421651133998182 1102 1 0.21472713282704348 1102 2 0.17778756594505296 1102 3 0.165238114306959 1102 4 0.11408680652330361 1102 5 0.06310236296184257 1102 6 0.10871183108302063 1102 7 0.05749396406164209 1102 8 0.11751650851770279 1102 9 0.14022304272881314 1102 10-11 0.1576381063101735 1102 12-13 0.2084161718880111 1102 14-15 0.15670530246568148 1102 16-17 0.21427157358061066 1102 18-19 0.0949885871856253 1102 20-21 0.08134789737943038 1102 22-23 0.0832095862214004 1102 24-25 0.0669196446804321 1102 26-27 0.08259532602738773 1102 28-29 0.10946144802839797 1102 30-31 0.1679273592634658 1102 32-33 0.15038123991190133 1102 34-35 0.1416052293097252 1102 36-37 0.12042620314229424 1102 38-39 0.12184079636295309 1102 40-41 0.04564043799455675 1102 42-43 0.010580377551129061 1102 44-45 -0.013758123559732383 1102 46-47 0.002137156172764776 1102 48-49 -0.046986725676283925 1102 50-51 -0.11313900151147038 1102 52-53 -0.09979342392610135 1102 54-55 -0.12960251869001382 1102 56-57 -0.10768818529819413 1102 58-59 -0.08857278631908372 1102 60-61 -0.09715689435820707 1102 62-63 -0.18274740472048023 1102 64-65 -0.1492104559806009 1102 66-67 -0.1912833811131165 1102 68-69 -0.23098176238292467 1102 70-71 -0.2851181771685134 1102 72-73 -0.304836949603974 1102 74-75 -0.4316593699343514 1102 76-77 -0.338852044169343 1102 78-79 -0.38762004091556435 1102 80-81 -0.30881891338306033 1102 82-83 -0.4438298578024664 1102 84-85 -0.37691600955789895 1102 86-87 -0.3415645713573632 1102 88-89 -0.4793584626091878 1102 90-91 -0.5042555849932064 1102 92-93 -0.6503041462291499 1102 94-95 -0.5417325331307126 1102 96-97 -0.5162222191286183 1102 98-99 -0.6823215553488033 1102 100 -0.5819885920256596 1103 1 0.27305762584036586 1103 2 0.24419475727869155 1103 3 0.20491063432548628 1103 4 0.18583481842666316 1103 5 0.18403452796838593 1103 6 0.13852449223615793 1103 7 0.1518483758810376 1103 8 0.17214159391835437 1103 9 0.14219445408000553 1103 10-11 0.16716718889445303 1103 12-13 0.2113259077917462 1103 14-15 0.20825876645883312 1103 16-17 0.22892495009699587 1103 18-19 0.25455929383176823 1103 20-21 0.21229824043404477 1103 22-23 0.158784642760331 1103 24-25 0.09718306334563209 1103 26-27 0.07900123858017594 1103 28-29 0.25213288745163 1103 30-31 0.3378581013078943 1103 32-33 0.2957255096236011 1103 34-35 0.22324392328218323 1103 36-37 0.18493636639319533 1103 38-39 0.1715717429104231 1103 40-41 0.18709703561214752 1103 42-43 0.20348969059107702 1103 44-45 0.25331576602798833 1103 46-47 0.34860384902641073 1103 48-49 0.3155771942608325 1103 50-51 0.22378982032378758 1103 52-53 0.176585124754439 1103 54-55 0.20207718797498586 1103 56-57 0.15813290663799506 1103 58-59 0.18396891034289098 1103 60-61 0.17674853801491963 1103 62-63 0.17006644809337246 1103 64-65 0.14364729824587386 1103 66-67 0.09163758360730156 1103 68-69 0.08252371726942442 1103 70-71 0.01773966876336175 1103 72-73 -0.011505002041126033 1103 74-75 0.0023529909345469946 1103 76-77 0.1123532959379645 1103 78-79 0.1783679803213687 1103 80-81 0.07549077676986826 1103 82-83 0.020866238145831772 1103 84-85 -0.009991497876413291 1103 86-87 0.026440280877217504 1103 88-89 -0.07212382364570402 1103 90-91 -0.0669115160144429 1103 92-93 -0.07670406736250968 1103 94-95 -0.053484325334430594 1103 96-97 -0.19417998573532103 1103 98-99 -0.1648558799156099 1103 100 -0.17832995416671693 1104 1 0.25198156604186295 1104 2 0.08896177657934601 1104 3 0.1417919647907624 1104 4 0.10579881272644087 1104 5 0.09202768737477385 1104 6 0.11024566096128297 1104 7 0.11731130417607716 1104 8 0.10352445534643806 1104 9 0.19570079531953155 1104 10-11 0.1640743000610314 1104 12-13 0.16863844064130262 1104 14-15 0.19786688565994126 1104 16-17 0.17317375867593654 1104 18-19 0.14034618505958463 1104 20-21 0.021116251767601568 1104 22-23 0.07049685492477664 1104 24-25 0.03509559467252643 1104 26-27 0.08929999181279413 1104 28-29 0.06799837834538636 1104 30-31 0.13464878942135527 1104 32-33 0.06565232767082563 1104 34-35 0.05703684455391311 1104 36-37 0.032915199109162074 1104 38-39 0.08009131098341271 1104 40-41 0.0494245490843781 1104 42-43 -0.038111562457324055 1104 44-45 -0.06380259652564746 1104 46-47 0.052261056923306626 1104 48-49 -0.05203313335514537 1104 50-51 -0.13525086785764984 1104 52-53 -0.035902071904615696 1104 54-55 0.003714180256402244 1104 56-57 0.09642885843026505 1104 58-59 -0.09348621780472399 1104 60-61 -0.18970914903350433 1104 62-63 -0.2064820186190346 1104 64-65 -0.12498100203873008 1104 66-67 -0.024300934253204787 1104 68-69 -0.17377894568953423 1104 70-71 -0.02204497136045802 1104 72-73 0.024776818016839286 1104 74-75 -0.055496799603197644 1104 76-77 -0.02399361824954127 1104 78-79 0.0801421216376248 1104 80-81 0.035788761870001906 1104 82-83 -0.0014370662377451993 1104 84-85 -0.16351514588847493 1104 86-87 -0.14622944309267538 1104 88-89 -0.11869420048742185 1104 90-91 -0.10491018014368692 1104 92-93 -0.18848161837126653 1104 94-95 -0.2259421670969637 1104 96-97 -0.39701383038657667 1104 98-99 -0.3652500542357302 1104 100 -0.35409579584550244 1105 1 0.3261034105530669 1105 2 0.1427757225664763 1105 3 0.14189790544918424 1105 4 0.11541630455084118 1105 5 0.12834284225110082 1105 6 0.0848496623228101 1105 7 0.09488751813717045 1105 8 0.13193289432100386 1105 9 0.16421299168354864 1105 10-11 0.12105817568711075 1105 12-13 0.18372972743242144 1105 14-15 0.20391802864421749 1105 16-17 0.1514350555614783 1105 18-19 0.13501691280768569 1105 20-21 0.10814399985456902 1105 22-23 0.10259721727610582 1105 24-25 0.028857347000048605 1105 26-27 0.09400249992988563 1105 28-29 0.18721737832591856 1105 30-31 0.23828611278845102 1105 32-33 0.25800137560373315 1105 34-35 0.18696724508411222 1105 36-37 0.1648388611277909 1105 38-39 0.1476308692481254 1105 40-41 0.16168947624885988 1105 42-43 0.17191162151865313 1105 44-45 0.2279746964164744 1105 46-47 0.2361631601665053 1105 48-49 0.1952431288883716 1105 50-51 0.21093643870064227 1105 52-53 0.16149227703095903 1105 54-55 0.10199348911098127 1105 56-57 0.07766857350086553 1105 58-59 0.1731092434826209 1105 60-61 0.20007829263514054 1105 62-63 0.10062996177689598 1105 64-65 0.15603492567641553 1105 66-67 0.164579122818985 1105 68-69 0.11980132697588886 1105 70-71 0.0864666051307097 1105 72-73 0.12267229483757802 1105 74-75 0.011919366936702147 1105 76-77 0.0266717987973486 1105 78-79 0.12710372984013674 1105 80-81 0.11496939281827778 1105 82-83 0.16223322833077347 1105 84-85 0.07887564060674634 1105 86-87 0.008807133519628252 1105 88-89 0.02474001120939917 1105 90-91 0.04268347665538741 1105 92-93 0.049394724675149604 1105 94-95 0.060515613390698775 1105 96-97 0.1580719634899772 1105 98-99 0.09720858776548269 1105 100 0.03962019286037588 1106 1 0.25068102449685625 1106 2 0.20206905348315019 1106 3 0.15704728034062754 1106 4 0.12816297906827856 1106 5 0.096860284020579 1106 6 0.09574603236946899 1106 7 0.0945644934242793 1106 8 0.11232323382617437 1106 9 0.09928747888547917 1106 10-11 0.14474973588342266 1106 12-13 0.1623446244854989 1106 14-15 0.19683948644067328 1106 16-17 0.2049326318177762 1106 18-19 0.12173706173817322 1106 20-21 0.17401691294772803 1106 22-23 0.1460226487944496 1106 24-25 0.1631576692793999 1106 26-27 0.23452038927267438 1106 28-29 0.17942298003155344 1106 30-31 0.3002398432645137 1106 32-33 0.2567556306295913 1106 34-35 0.2758757383867376 1106 36-37 0.2938182154012807 1106 38-39 0.25414344378165765 1106 40-41 0.3142176473477889 1106 42-43 0.34184739060469127 1106 44-45 0.3248171337886916 1106 46-47 0.2319425366069865 1106 48-49 0.2770241027305289 1106 50-51 0.21666247676910189 1106 52-53 0.19705258034046835 1106 54-55 0.20255709461351756 1106 56-57 0.3048757797464887 1106 58-59 0.37209917706878315 1106 60-61 0.2934396855456214 1106 62-63 0.31577754871698716 1106 64-65 0.2751432909986562 1106 66-67 0.20329751343217595 1106 68-69 0.22861966069214645 1106 70-71 0.3338212759295729 1106 72-73 0.24093760956981924 1106 74-75 0.1948787966315635 1106 76-77 0.2966297101071689 1106 78-79 0.34025349506342906 1106 80-81 0.29682896220978705 1106 82-83 0.25006756594557444 1106 84-85 0.22057424213379662 1106 86-87 0.22188326397045088 1106 88-89 0.30160769022132783 1106 90-91 0.3164206419963236 1106 92-93 0.1716678764890851 1106 94-95 0.1739149081677418 1106 96-97 0.21676833482918667 1106 98-99 0.1855626922971787 1106 100 0.10209805879946643 1107 1 0.20694851206194897 1107 2 0.16785922765190264 1107 3 0.1257989612097461 1107 4 0.11522682151269237 1107 5 0.04797120672993316 1107 6 0.09417627696493014 1107 7 0.06748540732296959 1107 8 0.09043223877009865 1107 9 0.1685081046028003 1107 10-11 0.08650775573833869 1107 12-13 0.13106555676668563 1107 14-15 0.13978879190583626 1107 16-17 0.18200240558481795 1107 18-19 0.16333749063653613 1107 20-21 0.1327359116670621 1107 22-23 0.23305372755201148 1107 24-25 0.2334492189920141 1107 26-27 0.20416082430564586 1107 28-29 0.23723610180038435 1107 30-31 0.27317857534782064 1107 32-33 0.14863159820200167 1107 34-35 0.18877199831646152 1107 36-37 0.24318707730605382 1107 38-39 0.27134613454081347 1107 40-41 0.2390732528453654 1107 42-43 0.22188301897574547 1107 44-45 0.2764902735441126 1107 46-47 0.18653845850442252 1107 48-49 0.2247504812664829 1107 50-51 0.23680345726737073 1107 52-53 0.2382437686394212 1107 54-55 0.23197997183398655 1107 56-57 0.23948997394786176 1107 58-59 0.2109972494322534 1107 60-61 0.1135826078261033 1107 62-63 0.216024897112284 1107 64-65 0.2558391653135388 1107 66-67 0.3431425800956234 1107 68-69 0.28249159775565147 1107 70-71 0.22084957531693306 1107 72-73 0.298395956175149 1107 74-75 0.260394861802304 1107 76-77 0.24024162098150015 1107 78-79 0.31097692913326824 1107 80-81 0.279585880603193 1107 82-83 0.30530370499202064 1107 84-85 0.2411180535727908 1107 86-87 0.18001241857068706 1107 88-89 0.16349539757795029 1107 90-91 0.0789441208426851 1107 92-93 0.09900506410459187 1107 94-95 0.22993322552813567 1107 96-97 0.3000214030172721 1107 98-99 0.396799548247845 1107 100 0.47398634446949117 1108 1 0.2410283742938617 1108 2 0.1411090473368617 1108 3 0.08625660090134346 1108 4 0.10076075246246319 1108 5 0.07176723267761531 1108 6 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0.04479039026218601 2316 44-45 -0.24727303152966584 2316 46-47 -0.04625419430215061 2316 48-49 -0.18783376259840168 2316 50-51 -0.030744318136932236 2316 52-53 -0.07692502842201066 2316 54-55 0.06634520583032355 2316 56-57 -0.033223651153491573 2316 58-59 -0.017775692847770586 2316 60-61 0.1139252471048593 2316 62-63 0.15698866566074798 2316 64-65 -0.1302219359681871 2316 66-67 -0.06604892713816213 2316 68-69 0.13695709442249182 2316 70-71 -0.11844266947682058 2316 72-73 -0.4281855128540002 2316 74-75 -0.03345868533875063 2316 76-77 -0.03269218509194616 2316 78-79 -0.3521053931720246 2316 80-81 -0.08319937438413305 2316 82-83 -0.0159676176535406 2316 84-85 0.11646298016315626 2316 86-87 0.1585310117999832 2316 88-89 0.25846319223052205 2316 90-91 0.21723561234848177 2316 92-93 0.391392880183826 2316 94-95 0.30825370489637294 2316 96-97 0.3876396406894216 2316 98-99 0.42089524326599204 2316 100 0.09270038738642583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 16.0 11 35.0 12 83.0 13 223.0 14 661.0 15 1657.0 16 3714.0 17 7529.0 18 13281.0 19 20909.0 20 29782.0 21 40872.0 22 53564.0 23 63405.0 24 69205.0 25 76832.0 26 88938.0 27 105471.0 28 125662.0 29 149913.0 30 178675.0 31 214897.0 32 260147.0 33 324627.0 34 421489.0 35 573027.0 36 821027.0 37 1254689.0 38 1264325.0 39 180631.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67845093402094 19.91686953724653 13.595744038812121 23.808935489920405 2 15.338767321626284 21.305529356469844 39.72608724453263 23.629616077371246 3 16.933061422614188 27.47099391352861 30.632334261065807 24.963610402791392 4 12.263332173932778 17.468562620159105 37.87949158052982 32.38861362537829 5 11.91335848171678 38.464166705540755 35.46676958844318 14.15570522429929 6 30.492003712379184 37.53137185775139 18.16022581906454 13.816398610804889 7 27.066165130570536 32.85906148576601 23.122131541016305 16.952641842647147 8 26.019387394395533 36.26839700897852 20.39055313283607 17.321662463789885 9 27.098425198091313 15.510914682444 20.480241614825196 36.910418504639495 10-11 24.40749504612441 26.360189208222263 29.32217617665528 19.910139568998048 12-13 24.940547593327018 24.162337476595503 29.436401665100785 21.46071326497669 14-15 22.34730069340239 25.66096305370955 26.772926851197315 25.218809401690745 16-17 22.10817198170048 28.787183212527438 27.117444194548312 21.987200611223766 18-19 21.875212512080928 27.873500201529417 29.174076106806258 21.077211179583397 20-21 22.560416036598312 26.919979493195733 29.126178782707147 21.39342568749881 22-23 22.239780259595065 26.841930620992166 28.904833660097218 22.013455459315555 24-25 21.978015397054627 26.9792883856086 29.115017254097104 21.927678963239668 26-27 22.287321282709513 27.011544158680294 29.05347701372688 21.647657544883312 28-29 21.940261694909914 27.373382136820496 28.972447568427977 21.71390859984161 30-31 22.45632184016736 26.994606284283996 29.033829238717622 21.515242636831022 32-33 22.239090852784134 26.932121439640692 29.04565420526134 21.783133502313827 34-35 22.164357155471066 27.43777629402669 28.921079090333624 21.476787460168627 36-37 22.150257954771018 27.294799069816772 29.033632738061982 21.521310237350225 38-39 22.288635591387884 27.422204633869118 28.602917397150684 21.686242377592315 40-41 22.44576713728989 27.100233266657575 28.864930480610006 21.589069115442527 42-43 22.042156908484973 27.305914992667653 28.749120496405702 21.90280760244168 44-45 22.071697003396807 27.48832741981445 28.58526783575296 21.854707741035785 46-47 22.124325758970336 27.39796075973946 28.64718218314139 21.830531298148816 48-49 22.11695675458773 27.34393740569135 28.523743163361576 22.015362676359345 50-51 22.145035868223946 27.48336613232405 28.41322993055198 21.958368068900025 52-53 21.905534385507945 27.6554816475427 28.45584577393762 21.983138193011733 54-55 22.011645713780474 27.76800047345041 28.258417239836124 21.961936572932995 56-57 22.176644174550255 27.717044405407815 28.034442031710448 22.071869388331482 58-59 22.026329069396898 27.751233461000318 28.211316856927347 22.011120612675438 60-61 22.38265341464266 27.67368063773756 28.07067868722469 21.872987260395085 62-63 21.963960563173828 27.831695648764633 28.2376801292127 21.966663658848837 64-65 22.043318216316525 27.838613335426377 28.224651651780153 21.89341679647695 66-67 21.985260057801657 27.733604253309124 28.147005713811986 22.134129975077233 68-69 21.77168763649936 27.48833135361972 28.31475145136966 22.425229558511255 70-71 22.127176299207424 27.28739450556603 28.36547910221634 22.219950093010198 72-73 21.83467071427333 27.954792268566898 27.713086473459647 22.497450543700122 74-75 21.91134082882465 28.018530788716163 27.734226748489156 22.335901633970035 76-77 21.865516154452326 27.56442868400315 28.142403467297083 22.427651694247437 78-79 21.936340118335266 27.725176882261728 28.029798577848887 22.30868442155412 80-81 21.76944046638033 28.003490066651544 27.735296790026016 22.491772676942116 82-83 22.0541468037176 27.782699074508994 27.859728752134984 22.303425369638422 84-85 22.144994520173288 28.021670241723218 27.601530489393255 22.23180474871024 86-87 22.045011249147556 28.07492790528633 27.615930214697272 22.264130630868838 88-89 21.821303607581875 27.68791838485271 27.814820427934624 22.675957579630797 90-91 21.848954901247357 27.679407515681053 28.06868291766273 22.40295466540886 92-93 21.859268658456298 27.81912615859561 27.942358161619694 22.379247021328396 94-95 21.72562614725614 27.642078470270498 27.74730601715548 22.884989365317885 96-97 21.553551919545193 27.85647778821787 28.006387949306866 22.583582342930075 98-99 21.843160339346152 27.686401698322776 27.777296581420828 22.693141380910244 100 20.981577298502934 28.178970141431176 27.929903098560466 22.90954946150542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3435.0 1 2525.0 2 3172.0 3 4387.5 4 2907.0 5 2114.0 6 3208.5 7 6106.0 8 9759.0 9 11252.5 10 9245.5 11 6867.0 12 6568.0 13 6946.5 14 7519.5 15 7899.5 16 8174.5 17 8680.5 18 9338.0 19 9829.5 20 10481.0 21 11763.5 22 13535.0 23 16208.0 24 20184.5 25 25029.0 26 30750.5 27 37636.5 28 45889.0 29 54992.5 30 65417.0 31 76118.5 32 87273.5 33 100453.0 34 114250.0 35 129101.0 36 148940.5 37 170730.5 38 189829.5 39 208619.0 40 220328.5 41 223640.5 42 229662.5 43 237825.0 44 247542.0 45 261407.5 46 270174.5 47 272491.0 48 279567.0 49 282077.0 50 269397.5 51 250881.5 52 233412.0 53 213841.0 54 190874.0 55 167486.0 56 147940.0 57 130597.5 58 113894.0 59 95688.0 60 75800.5 61 60446.0 62 47780.5 63 36044.0 64 26123.5 65 19057.5 66 14061.5 67 10427.5 68 7799.0 69 5928.5 70 4541.5 71 3559.0 72 2661.0 73 1905.5 74 1442.0 75 1082.0 76 783.5 77 502.0 78 346.5 79 247.5 80 172.5 81 119.0 82 84.5 83 64.5 84 50.5 85 38.5 86 24.5 87 15.5 88 10.5 89 9.0 90 9.0 91 5.5 92 5.5 93 4.5 94 3.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24801010384684366 2 0.07826257709242077 3 0.0013553325875852165 4 0.001150456731322335 5 5.358291625336903E-4 6 7.722243812985536E-4 7 0.0 8 7.722243812985536E-4 9 0.0 10-11 3.388331468963041E-4 12-13 0.001646886690728548 14-15 7.879840625495445E-6 16-17 4.727904375297267E-5 18-19 0.0012371349782027848 20-21 0.0014971697188441346 22-23 7.879840625495445E-6 24-25 2.3639521876486336E-5 26-27 2.994339437688269E-4 28-29 0.0020645182438798065 30-31 0.0022693941001426885 32-33 0.003073137843943224 34-35 0.004357551865898981 36-37 0.001646886690728548 38-39 0.0031204168876961965 40-41 0.00664270564729266 42-43 0.012891419263310548 44-45 0.008226553613017244 46-47 0.00620931441289041 48-49 0.007060337200443919 50-51 0.005429210190966362 52-53 0.004042358240879163 54-55 0.006248713616017887 56-57 0.004223594575265558 58-59 0.0023954715501506153 60-61 0.008896340066184357 62-63 0.011480927791346863 64-65 0.007399170347340223 66-67 0.0079586390317504 68-69 0.015034735913445308 70-71 0.0047751834190502395 72-73 0.012859899900808566 74-75 0.008100476163009318 76-77 0.004822462462803212 78-79 0.016445227385408996 80-81 0.006957899272312478 82-83 0.01572816188848891 84-85 0.024955455260944077 86-87 0.016508266110412957 88-89 0.016429467704158002 90-91 0.031235688239463943 92-93 0.024908176217191103 94-95 0.030100991189392603 96-97 0.018486106107412314 98-99 0.01835214881677889 100 0.019400167619969787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6345306.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.48602532591918 #Duplication Level Percentage of deduplicated Percentage of total 1 72.68989267234164 42.5134290377292 2 16.497467039517293 19.297425501734505 3 5.350333924427846 9.387592962186348 4 2.163509884924715 5.061403756903335 5 1.0540075352981035 3.0822355701577266 6 0.5737399681899104 2.0133462186028295 7 0.3698935172184464 1.5143521131151965 8 0.24858340815979252 1.1630924404189544 9 0.18098715903806564 0.9526697610449824 >10 0.8004150005036662 8.126319389541523 >50 0.04442725640640377 1.7818745182621725 >100 0.024650143931138718 2.635172866836043 >500 0.0014895700304223716 0.586912379198848 >1k 4.610564801618209E-4 0.5536196034088483 >5k 3.5465883089119774E-5 0.12675793916895842 >10k+ 1.0639764926735932E-4 1.2037959416904764 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23246 0.36634955036053424 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20426 0.32190724923273994 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14363 0.22635630180798216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.575968125099089E-5 2 0.0 0.0 0.0 0.0 1.575968125099089E-5 3 0.0 0.0 0.0 0.0 1.575968125099089E-5 4 0.0 0.0 0.0 0.0 1.575968125099089E-5 5 0.0 0.0 0.0 0.0 1.575968125099089E-5 6 0.0 0.0 0.0 3.151936250198178E-5 3.151936250198178E-5 7 0.0 0.0 0.0 3.151936250198178E-5 3.151936250198178E-5 8 0.0 0.0 0.0 3.151936250198178E-5 3.151936250198178E-5 9 0.0 0.0 0.0 7.879840625495445E-5 3.151936250198178E-5 10-11 0.0 0.0 0.0 7.879840625495445E-5 3.151936250198178E-5 12-13 0.0 0.0 0.0 9.455808750594535E-5 3.151936250198178E-5 14-15 0.0 1.575968125099089E-5 0.0 9.455808750594535E-5 4.727904375297267E-5 16-17 1.575968125099089E-5 3.151936250198178E-5 0.0 1.1819760938243168E-4 6.303872500396356E-5 18-19 1.575968125099089E-5 3.151936250198178E-5 0.0 1.2607745000792713E-4 7.879840625495445E-5 20-21 1.575968125099089E-5 3.151936250198178E-5 0.0 1.8123633438639524E-4 7.879840625495445E-5 22-23 1.575968125099089E-5 3.151936250198178E-5 0.0 2.3639521876486335E-4 9.455808750594535E-5 24-25 1.575968125099089E-5 3.151936250198178E-5 0.0 4.0975171252576314E-4 9.455808750594535E-5 26-27 1.575968125099089E-5 3.151936250198178E-5 0.0 5.909880469121584E-4 9.455808750594535E-5 28-29 1.575968125099089E-5 3.151936250198178E-5 0.0 0.0017572044594854844 1.0243792813144078E-4 30-31 1.575968125099089E-5 3.151936250198178E-5 0.0 0.005728644134735188 1.1031776875693622E-4 32-33 1.575968125099089E-5 3.151936250198178E-5 0.0 0.014018236472756396 1.2607745000792713E-4 34-35 1.575968125099089E-5 3.151936250198178E-5 0.0 0.0257749586859956 1.41837131258918E-4 36-37 1.575968125099089E-5 3.151936250198178E-5 0.0 0.04413498734339999 1.41837131258918E-4 38-39 1.575968125099089E-5 3.151936250198178E-5 0.0 0.0728491265827054 1.575968125099089E-4 40-41 1.575968125099089E-5 3.151936250198178E-5 0.0 0.10785925848178166 1.575968125099089E-4 42-43 1.575968125099089E-5 3.151936250198178E-5 0.0 0.13899250879311414 1.733564937608998E-4 44-45 1.575968125099089E-5 3.151936250198178E-5 0.0 0.17590168228293482 1.733564937608998E-4 46-47 1.575968125099089E-5 3.151936250198178E-5 0.0 0.2193590033325422 2.2063553751387247E-4 48-49 1.575968125099089E-5 3.151936250198178E-5 0.0 0.2657870242979613 2.679145812668451E-4 50-51 3.151936250198178E-5 3.151936250198178E-5 0.0 0.3153118226292002 2.679145812668451E-4 52-53 3.151936250198178E-5 3.151936250198178E-5 0.0 0.3663022713167813 2.7579442189234056E-4 54-55 3.151936250198178E-5 3.151936250198178E-5 0.0 0.4200585440639112 2.83674262517836E-4 56-57 3.151936250198178E-5 3.151936250198178E-5 0.0 0.47933858508951344 2.83674262517836E-4 58-59 3.151936250198178E-5 3.151936250198178E-5 0.0 0.5500443950220841 2.83674262517836E-4 60-61 3.151936250198178E-5 3.151936250198178E-5 0.0 0.6220109794547339 2.83674262517836E-4 62-63 3.151936250198178E-5 3.151936250198178E-5 0.0 0.6976968486626176 2.83674262517836E-4 64-65 3.151936250198178E-5 3.151936250198178E-5 0.0 0.7694979564421321 3.151936250198178E-4 66-67 3.151936250198178E-5 3.151936250198178E-5 0.0 0.8523859999817187 3.151936250198178E-4 68-69 3.151936250198178E-5 3.151936250198178E-5 0.0 0.9569593649226689 3.3095330627080867E-4 70-71 3.151936250198178E-5 3.151936250198178E-5 0.0 1.0619740009386467 3.624726687727905E-4 72-73 3.151936250198178E-5 3.151936250198178E-5 0.0 1.1583996106728343 4.412710750277449E-4 74-75 3.151936250198178E-5 3.151936250198178E-5 0.0 1.2574570871759376 4.570307562787358E-4 76-77 3.151936250198178E-5 3.151936250198178E-5 0.0 1.3662934458952807 4.6491059690423125E-4 78-79 3.151936250198178E-5 3.151936250198178E-5 0.0 1.4856572716902856 4.727904375297267E-4 80-81 3.151936250198178E-5 3.151936250198178E-5 0.0 1.606108515491609 4.885501187807176E-4 82-83 3.151936250198178E-5 3.151936250198178E-5 0.0 1.7330212286058386 5.043098000317085E-4 84-85 3.151936250198178E-5 3.151936250198178E-5 0.0 1.8647721638641226 5.043098000317085E-4 86-87 3.151936250198178E-5 3.151936250198178E-5 0.0 1.9953174835067056 5.12189640657204E-4 88 3.151936250198178E-5 3.151936250198178E-5 0.0 2.099993286375787 5.200694812826994E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10580 0.0 48.285076 1 TACCTGG 5485 0.0 38.6636 2 GTATCAA 17995 0.0 36.743042 1 GTACCTG 5800 0.0 36.726517 1 ACCTGGG 6480 0.0 32.413822 3 TCAACGC 22525 0.0 28.99573 4 ATCAACG 22640 0.0 28.87011 3 CAACGCA 22645 0.0 28.842302 5 GTACATG 20605 0.0 28.063461 1 TATCAAC 23670 0.0 27.752401 2 AACGCAG 23635 0.0 27.7148 6 TATAACG 515 0.0 26.478489 2 TACATGG 22005 0.0 25.792196 2 ACATGGG 21650 0.0 25.415386 3 ACGCAGA 27000 0.0 24.156101 7 CGCAGAG 27605 0.0 23.47349 8 ATGGGGG 7000 0.0 23.158398 5 CATGGGG 14760 0.0 23.111828 4 TAACGCA 575 0.0 22.880959 4 ATAACGC 680 0.0 22.803566 3 >>END_MODULE