FastQCFastQC Report
Mon 6 Jun 2016
ERR1211624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5376940
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177060.3292951009310128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158040.2939218217052822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103190.19191212845968153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73440.13658326111133842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84050.050.008641
TACCTGG62850.045.2086522
GTACCTG66300.043.616621
ACCTGGG74350.037.7941863
GTATCAA139250.036.4859431
TATAACG5600.032.7617032
TCAACGC165100.029.8555554
ATCAACG167850.029.4509413
CAACGCA168600.029.2060075
TATCAAC176900.028.3477952
AACGCAG176900.027.9106926
TAACGCA6950.027.7201634
GTACATG151000.026.8049761
ATAACGC6850.026.0674063
TACATGG155150.025.28742
CCTGGGG72600.025.2420564
ACGCAGA202950.024.189287
ACATGGG157500.024.1364153
CGCAGAG208000.023.4226178
CATGGGG101050.022.1343784
Produced by FastQC (version 0.11.5)