FastQCFastQC Report
Mon 6 Jun 2016
ERR1211624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5376940
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208320.38743225700863315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186820.34744668900899023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128590.23915089251507365No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75200.1398564983057278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98950.047.6604231
TACCTGG63250.044.9079972
GTACCTG65850.043.8297841
GTATCAA162400.036.7348441
ACCTGGG77550.035.8114363
TCAACGC201550.029.143064
CAACGCA205350.028.6492655
ATCAACG205650.028.6082733
TATAACG6650.028.2869192
TATCAAC215000.027.5162662
AACGCAG215300.027.412816
CCTGGGG67400.027.1204974
TAACGCA6800.026.95034
GTACATG170400.024.3549231
ATAACGC7850.023.9445323
ACGCAGA246350.023.880957
CGCAGAG250800.023.3635468
TACATGG173850.023.2091852
ACATGGG173400.022.3844763
CATGGGG107000.021.4310764
Produced by FastQC (version 0.11.5)