FastQCFastQC Report
Mon 6 Jun 2016
ERR1211623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161430.45309944947701153No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134060.37627771911595226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92590.2598803074216472No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82680.23206505905196875No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.13079622341342215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62650.063.2348822
GTACCTG63900.062.129791
ACCTGGG72050.053.9985853
TAACGCA5850.045.7843634
GGTATCA84650.040.9958081
CCTGGGG56350.039.9429174
TATAACG6300.039.58232
GTATCAA138600.034.8697851
ATAACGC8850.030.2633933
TATCACG3650.029.648362
TCAACGC168300.028.0874334
ATCAACG170000.027.805783
CAACGCA169950.027.6990875
AACGCAG177050.026.929116
TATCAAC176600.026.8023552
CTGGGGG38000.026.2102535
GTACATG148300.025.4035421
GTATAAC12550.025.1721171
TACATGG151150.024.4047072
GTACCCG5300.024.0201971
Produced by FastQC (version 0.11.5)