FastQCFastQC Report
Mon 6 Jun 2016
ERR1211623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562794
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189650.5323069478617063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161620.45363273879994187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119760.33614068060067465No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86890.2438816277337393No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46770.13127337701814923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG63300.062.631432
GTACCTG65650.060.8973731
ACCTGGG69400.055.9240723
TAACGCA6750.045.2461554
TATAACG6900.044.302962
CCTGGGG54250.042.092884
GGTATCA98150.037.8506471
ATAACGC8850.037.16493
GTATCAA156900.033.4132961
GTATAAC12850.031.1855371
GTACCCG5250.030.5321251
CTGGGGG35250.028.7920675
TCAACGC190750.027.0217954
ATCAACG191800.026.8252473
CAACGCA192900.026.8184285
TATCAAC196800.026.2866972
AACGCAG201150.025.953546
TATCACG4250.025.4511492
GTACATG153700.024.4774671
CATGGGG71850.023.8692194
Produced by FastQC (version 0.11.5)