FastQCFastQC Report
Mon 6 Jun 2016
ERR1211622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209874
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206920.2869953067140979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175150.2429307363762529No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108520.15051580651756188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103400.048.2422181
GTATCAA180300.036.975651
TCAACGC210400.030.86614
CAACGCA211350.030.700445
ATCAACG212600.030.4142883
AACGCAG219850.029.4880146
TATCAAC226250.028.7982442
GTACATG224350.028.0134261
GTACCTG59200.027.5560231
TACCTGG58350.027.0896422
TACATGG227400.027.038962
ACATGGG227750.025.8119833
ACGCAGA251600.025.5619967
CGCAGAG258650.024.5583578
CATGGGG135650.022.8981864
GCAGAGT289250.021.164269
GAGTACT188600.019.84343512-13
GAGTACA163600.019.5680981
AGAGTAC265550.019.1534410-11
ACCTGGG80050.019.0785123
Produced by FastQC (version 0.11.5)