FastQCFastQC Report
Mon 6 Jun 2016
ERR1211622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209874
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230170.31924274959590143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211210.2929454800458371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136950.18994784097475212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117850.048.396851
GTATCAA196600.036.0360151
TCAACGC235050.029.5273194
ATCAACG237450.029.2092883
CAACGCA238550.029.1531875
AACGCAG249350.027.9470236
TATCAAC255850.027.351042
GTACATG242450.027.0825941
GTACCTG60000.026.9466111
TACATGG246100.025.9504242
TACCTGG61600.025.2699052
ACATGGG245600.025.0830943
ACGCAGA289150.023.9547
CATGGGG148050.023.6774084
CGCAGAG294300.023.5348268
GCAGAGT329900.020.595619
ATGGGGG86150.020.4540715
TAACGCA6700.020.3388754
GAGTACT210000.019.58919112-13
AGAGTAC305100.019.00472610-11
Produced by FastQC (version 0.11.5)