FastQCFastQC Report
Mon 6 Jun 2016
ERR1211621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227025
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138060.3266126885930412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115610.2735020493136426No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79260.18750776255167642No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56090.13269379764728148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG49900.046.814551
TACCTGG49750.046.6368452
GGTATCA70250.045.6813351
ACCTGGG60200.037.6260033
GTATCAA118400.036.3113941
TCAACGC140450.030.0780584
ATCAACG141400.029.811283
CAACGCA143200.029.5632725
TATCAAC147950.028.8832592
AACGCAG148600.028.7361416
GTACATG127750.027.4475841
TATAACG4500.026.1458532
TACATGG130250.025.9792422
ACGCAGA167900.025.237097
TAACGCA4850.025.1908874
CCTGGGG53750.025.1782684
ACATGGG132050.024.7334063
CGCAGAG173000.024.2507258
CATGGGG80500.024.1666414
ATAACGC5350.022.837953
Produced by FastQC (version 0.11.5)