FastQCFastQC Report
Mon 6 Jun 2016
ERR1211621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227025
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158800.3756779295130736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140810.3331184461885132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99280.23486967784671253No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58050.13733062851532699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG53350.050.1631742
GTACCTG56450.047.9493561
GGTATCA77900.046.913631
ACCTGGG62450.041.909113
GTATCAA127300.035.7094761
TATAACG5200.034.3706252
TAACGCA4900.033.562364
CCTGGGG55200.030.9843834
TCAACGC155600.028.5366364
ATCAACG156150.028.4966453
CAACGCA157950.028.290225
ATAACGC6300.027.5960463
TATCAAC164000.027.2736852
AACGCAG165450.027.1233186
GTACATG136700.024.6989971
TATCACG3850.024.432982
TACATGG137200.024.0652392
ACGCAGA187600.023.7950577
ACATGGG134350.023.6425723
CGCAGAG192550.023.110138
Produced by FastQC (version 0.11.5)