FastQCFastQC Report
Mon 6 Jun 2016
ERR1211620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6963277
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221090.3175085523669387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190220.2731759773451494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125790.18064770366021632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81500.11704259359494101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.046.367971
GTACCTG75850.041.532521
TACCTGG79050.039.1036722
GTATCAA185100.036.0510061
ACCTGGG98650.031.4350383
TCAACGC217050.029.765934
ATCAACG218550.029.5183
CAACGCA221300.029.1478425
TATCAAC231600.028.258082
AACGCAG230500.028.1450866
GTACATG220450.028.02391
TACATGG222550.026.9336932
ACATGGG222500.025.8263853
TAACGCA6800.024.8754044
ACGCAGA264800.024.3399187
CATGGGG138450.023.9940194
CGCAGAG269700.023.7064428
TATAACG6650.023.3478222
ATAACGC8500.021.5582183
CCTGGGG86050.020.9679954
Produced by FastQC (version 0.11.5)