FastQCFastQC Report
Mon 6 Jun 2016
ERR1211620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6963277
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264170.37937597484632596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230910.33161110781604697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161450.2318592237534138No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84220.12094880039958199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119300.045.4936871
TACCTGG75750.044.019212
GTACCTG77450.043.5312231
GTATCAA203750.036.4038541
ACCTGGG94800.033.955123
TATAACG7550.032.39182
TCAACGC248850.029.344734
TAACGCA8550.029.1290634
ATCAACG251250.028.9900283
CAACGCA253350.028.8235135
TATCAAC263700.027.911482
AACGCAG264850.027.6790246
GTACATG240400.027.087911
TACATGG241500.026.4655552
ATAACGC9750.026.0262853
ACATGGG240550.025.5520293
CATGGGG143400.024.3803844
CCTGGGG85700.023.9616874
ACGCAGA304550.023.8852127
CGCAGAG311000.023.3596258
Produced by FastQC (version 0.11.5)