FastQCFastQC Report
Mon 6 Jun 2016
ERR1211619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12626
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCTTAAAGAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAA940.7444954855060985No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC580.4593695548867417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.22176461270394426No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.20592428322509107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.1900839537462379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.1188024710913987No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG150.1188024710913987No Hit
GAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAATACATTTACA150.1188024710913987No Hit
GTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTG140.11088230635197213No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA130.10296214161254554No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC130.10296214161254554No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT301.307952E-462.7755621
TTCTGAA250.00508148656.4980052
CTTACAC250.00508148656.4980053
ACTTTTT405.4170506E-447.0816733
TACTTTT405.4170506E-447.0816732
CTTTTTT500.001624776537.6653374
Produced by FastQC (version 0.11.5)