FastQCFastQC Report
Mon 6 Jun 2016
ERR1211618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432282
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231730.3117884924172683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200930.27034765365469177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133360.17943344991484447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113650.047.1681631
GTATCAA189850.037.275891
TACCTGG67000.033.769872
GTACCTG74000.031.474091
TCAACGC230250.029.9783344
ATCAACG233350.029.620953
CAACGCA233950.029.4809595
AACGCAG241750.028.5461246
TATCAAC244700.028.3157372
GTACATG227200.027.9522781
TACATGG236200.026.3076482
ACGCAGA274050.024.907697
ACATGGG240250.024.8390183
ACCTGGG90150.024.3935093
CGCAGAG280150.024.2157338
CATGGGG145850.023.0670434
GCAGAGT312500.021.047439
AGAGTAC284700.019.48984110-11
GAGTACT197400.019.30341712-13
CAGAGTA304450.018.649910-11
Produced by FastQC (version 0.11.5)