FastQCFastQC Report
Mon 6 Jun 2016
ERR1211618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432282
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256570.34521025978293074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233140.3136856217242564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157910.2124650275648852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128150.048.2985041
GTATCAA212700.035.6817361
GTACCTG68400.031.7021921
TACCTGG67750.031.2346652
TCAACGC259500.028.6095734
ATCAACG262300.028.3403843
CAACGCA263200.028.2073885
TATCAAC272250.027.5503062
AACGCAG273600.027.222336
GTACATG243850.026.0394861
TACATGG250500.025.2304612
ACATGGG249150.024.5742933
ACGCAGA308950.023.985887
ACCTGGG86250.023.9167923
CATGGGG153150.023.2257924
CGCAGAG318800.023.1268658
TAACGCA8150.021.3322074
ATGGGGG89300.021.257965
GCAGAGT352250.020.5438529
GAGTACT222000.020.34120612-13
Produced by FastQC (version 0.11.5)