FastQCFastQC Report
Mon 6 Jun 2016
ERR1211617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805612
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188810.2774328010471358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169430.24895630253385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110290.1620574314257116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99500.045.9404951
GTATCAA163350.038.2352371
GTACCTG69900.037.5900541
TACCTGG69700.036.7202642
TCAACGC197550.030.6860754
CAACGCA199350.030.3591775
ATCAACG200150.030.1926543
ACCTGGG85200.029.5624963
AACGCAG208550.029.0594166
GTACATG205800.028.9961361
TATCAAC210750.028.7756442
TACATGG211700.027.401982
TATAACG7600.027.2382242
ACATGGG210550.026.4025213
ACGCAGA237200.025.3714357
TAACGCA7600.025.3512234
CGCAGAG244150.024.5727838
CATGGGG143350.024.4879024
ATAACGC9200.021.4524753
GCAGAGT270950.021.240469
Produced by FastQC (version 0.11.5)