FastQCFastQC Report
Mon 6 Jun 2016
ERR1211617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805612
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220000.32326262502182024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191110.2808123648541821No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135590.19923263330322094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109050.046.0870061
GTACCTG71650.038.2302321
TACCTGG72200.036.995482
GTATCAA184100.035.9550821
ACCTGGG87450.029.768623
TCAACGC224650.028.8861474
CAACGCA228250.028.4719335
ATCAACG228700.028.2722913
AACGCAG239450.027.239116
TATCAAC238400.027.2213942
GTACATG222300.026.977061
TACATGG226350.025.8866042
ACATGGG227500.024.7448123
CATGGGG147450.023.8374164
ACGCAGA275800.023.6149647
CGCAGAG282800.022.9805858
TATAACG6850.021.9683252
ATGGGGG85850.020.6351015
ATAACGC8300.020.381363
CCTGGGG85750.020.2754274
Produced by FastQC (version 0.11.5)