FastQCFastQC Report
Mon 6 Jun 2016
ERR1211616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205415
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261330.2838872554903826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228370.24808224289725123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144590.15707059377551147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147900.047.7013781
GTATCAA254150.036.146511
GTACATG334450.030.0482921
TCAACGC300500.029.3190524
CAACGCA302400.029.1177165
ATCAACG304600.028.9547923
AACGCAG315450.028.1322776
TACATGG352450.028.097772
ACATGGG351450.027.24753
TATCAAC327050.027.1584592
TACCTGG77000.026.63872
CATGGGG211800.025.5575754
GTACCTG84600.024.9732571
ACGCAGA355900.024.6975567
CGCAGAG367900.023.7135938
ATGGGGG121500.020.9987325
TATAACG8250.020.5289062
GCAGAGT414100.020.398579
GAGTACT234750.020.15565712-13
ACCTGGG100600.019.6172143
Produced by FastQC (version 0.11.5)