FastQCFastQC Report
Mon 6 Jun 2016
ERR1211616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205415
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289800.31481470417140345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252690.27450147549024134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174430.18948629692414737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165300.044.407711
GTATCAA274050.035.2840581
GTACATG360050.028.7942031
GTACCTG80200.028.4520571
TACCTGG77350.028.2074222
TCAACGC335950.027.9034394
ATCAACG337800.027.7231043
CAACGCA338950.027.6287425
TACATGG377100.027.0214142
AACGCAG350050.026.7940796
ACATGGG374100.026.4900683
TATCAAC363550.025.9590572
CATGGGG217050.025.3288564
TATAACG7900.024.4040812
ACGCAGA398300.023.3712837
ATGGGGG118950.022.7929675
CGCAGAG410150.022.6043878
ACCTGGG97450.022.1804433
GCAGAGT454950.020.0686199
GAGTACT257800.019.44866812-13
Produced by FastQC (version 0.11.5)