FastQCFastQC Report
Mon 6 Jun 2016
ERR1211615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7814863
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263430.33708844288121237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219460.2808238608917392No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150200.19219786706433625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125300.046.0692631
GTATCAA214700.037.913131
GTACCTG74700.036.6174661
TACCTGG73500.036.4822652
TCAACGC257800.030.4589584
ATCAACG259250.030.41473
CAACGCA260300.030.2355335
AACGCAG269950.029.2027996
ACCTGGG87300.029.1739623
TATCAAC272450.029.0942632
GTACATG252950.028.7495211
TACATGG259900.027.5849972
ACATGGG255950.026.4740543
ACGCAGA303500.025.7735467
CGCAGAG310750.024.9008968
CATGGGG159900.024.2746524
TATAACG8600.024.0684722
TAACGCA6900.023.8363864
GCAGAGT346400.021.687049
ATGGGGG98650.021.2905775
Produced by FastQC (version 0.11.5)