FastQCFastQC Report
Mon 6 Jun 2016
ERR1211615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7814863
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289120.36996169990440014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242120.3098198906365985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179050.2291147010510613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138550.047.0886651
GTATCAA233350.036.9683531
TACCTGG75600.036.0149462
GTACCTG79350.034.8126831
TCAACGC289400.029.0966554
ACCTGGG92150.029.0150493
ATCAACG291950.028.8587933
CAACGCA294350.028.623315
AACGCAG306500.027.596856
TATCAAC308350.027.5422762
TAACGCA8750.027.388344
GTACATG270150.026.0171361
TATAACG9950.025.0483322
TACATGG279100.024.7338812
ACATGGG271300.024.2311653
ACGCAGA348400.024.1429067
CGCAGAG357750.023.4069888
CATGGGG166700.022.6915324
ATAACGC11300.021.6237643
GCAGAGT395350.020.8477769
Produced by FastQC (version 0.11.5)