FastQCFastQC Report
Mon 6 Jun 2016
ERR1211614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10763904
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262750.24410288311750086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225500.20949648008752214No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145930.13557348709167233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142450.048.0131531
GTATCAA251050.038.0093421
TCAACGC291750.031.3750764
CAACGCA293950.031.073145
ATCAACG295250.031.0500283
AACGCAG305850.030.0437456
TATCAAC319850.028.8304652
GTACATG321750.028.411221
TACATGG333750.026.7275372
ACATGGG334200.025.589373
ACGCAGA356550.025.5347217
CGCAGAG365050.024.8252588
TACCTGG82300.024.1242892
CATGGGG192750.024.0131194
GTACCTG87450.023.6248471
GCAGAGT416150.020.9075419
GAGTACT254950.019.59036812-13
AGAGTAC372450.019.10515410-11
GAGTACA239300.018.9621281
ATGGGGG118600.018.8575215
Produced by FastQC (version 0.11.5)