FastQCFastQC Report
Mon 6 Jun 2016
ERR1211614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10763904
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304280.2826855386298503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263380.24468817261841055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180580.16776440964170622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155850.047.342871
GTATCAA267050.037.3744961
TCAACGC323300.030.114534
ATCAACG324600.029.9940623
CAACGCA328750.029.6152945
AACGCAG341200.028.5769146
TATCAAC351400.027.9172062
GTACATG339200.027.3815121
TACATGG348250.026.1711032
ACATGGG342900.025.4334093
ACGCAGA396400.024.4905687
GTACCTG86450.024.1048451
TACCTGG82750.023.9261882
CGCAGAG406750.023.7518658
CATGGGG205600.023.1286074
GCAGAGT460700.020.5216339
GAGTACT282450.019.55582612-13
AGAGTAC416750.019.26941910-11
ATGGGGG118900.018.8507485
CAGAGTA448650.018.00405710-11
Produced by FastQC (version 0.11.5)