FastQCFastQC Report
Mon 6 Jun 2016
ERR1211613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777327
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141080.2441959750590541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116700.20199652884456773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.1392685579334526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76750.044.7404981
GTACCTG67150.042.9885941
TACCTGG66900.042.4748152
GTATCAA132300.036.507991
ACCTGGG78850.035.757413
TCAACGC149800.031.5575054
CAACGCA152950.031.088155
ATCAACG151850.031.0695783
AACGCAG160900.029.7226736
TATCAAC166200.028.3916452
GTACATG166300.027.7392331
TAACGCA6050.027.1849864
TATAACG6350.026.6716232
TACATGG167750.026.334422
ACGCAGA181200.026.1856357
CGCAGAG186600.025.227758
ACATGGG170550.025.1832033
CCTGGGG75350.024.6961044
ATAACGC7200.024.1482523
CATGGGG105800.022.8737854
Produced by FastQC (version 0.11.5)