FastQCFastQC Report
Mon 6 Jun 2016
ERR1211613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5777327
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161830.2801122387567815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136820.23682232284930382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99290.17186148542396856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG70150.045.2928161
TACCTGG68850.045.0825842
GGTATCA90000.043.107661
GTATCAA153800.038.0682031
ACCTGGG82700.036.818183
TCAACGC184550.030.7308644
ATCAACG187550.030.2651393
CAACGCA188200.030.0604825
TATCAAC196150.029.1790472
AACGCAG195550.029.075046
TAACGCA6500.027.4695634
GTACATG183950.026.1138841
TACATGG186850.025.0185492
ACGCAGA225300.024.9855217
CCTGGGG74300.024.916614
ACATGGG181350.024.8222273
TATAACG6450.024.7906572
CGCAGAG230450.024.3455968
CATGGGG106200.022.7857994
ATAACGC7350.021.7362383
Produced by FastQC (version 0.11.5)