FastQCFastQC Report
Mon 6 Jun 2016
ERR1211612_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211612_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10516381
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282390.2685239342317476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250680.23837097571873822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162920.1549202144730207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146700.050.14841
GTATCAA242550.036.7276651
TCAACGC294700.029.3211634
CAACGCA296800.029.2053555
ATCAACG296500.029.1756863
GTACATG319300.028.0766871
AACGCAG311300.027.8267366
TATCAAC319550.027.297512
TACATGG325600.026.8914432
TACCTGG88300.026.3752522
GTACCTG91450.026.3511661
ACATGGG325550.025.9804553
ACGCAGA357200.024.0145177
CGCAGAG365900.023.2002538
CATGGGG190900.022.5213664
ACCTGGG114400.019.9208413
GCAGAGT413250.019.848539
GAGTACT257250.019.83495912-13
GAGTACA233450.019.1503661
AGAGTAC374400.018.63466810-11
Produced by FastQC (version 0.11.5)