FastQCFastQC Report
Mon 6 Jun 2016
ERR1211612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10516381
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319110.3034408890282693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288040.2738965048907985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199660.189856187218778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163550.047.4280781
GTATCAA271100.036.0222431
TCAACGC331300.028.6932934
ATCAACG333050.028.5004713
CAACGCA334700.028.4011445
AACGCAG346200.027.5126636
GTACATG333400.027.4523851
TATCAAC357850.026.8084562
GTACCTG87250.026.6441351
TACCTGG86650.026.3218172
TACATGG339050.026.228322
ACATGGG335350.025.6986963
ACGCAGA400700.023.700147
CATGGGG192550.023.1838764
CGCAGAG409200.023.127458
GTATAGG40550.020.5827081
ACCTGGG110050.020.2821353
GCAGAGT464050.020.029199
GAGTACT291350.019.74907112-13
AGAGTAC423500.018.91798610-11
Produced by FastQC (version 0.11.5)