FastQCFastQC Report
Mon 6 Jun 2016
ERR1211611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548437
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203020.2689563415578616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174110.2306570221093453No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116570.1544293209309424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.048.2035061
TACCTGG66550.040.6458322
GTACCTG72550.038.409491
GTATCAA175850.037.189691
TATAACG6150.035.1867332
ACCTGGG82350.032.2959483
GTACATG226200.030.1831421
TCAACGC213000.029.6491344
ATCAACG216750.029.1580453
CAACGCA217600.028.975335
TATCAAC224650.028.3745572
AACGCAG230100.027.520686
TACATGG245350.027.131052
ACATGGG251300.025.7288953
TAACGCA7650.025.183424
ATAACGC8550.023.631843
CGCAGAG261800.023.418158
GTCCTAC48100.022.546091
ACGCAGA281450.022.2493657
ATGGGGG76450.022.2467125
Produced by FastQC (version 0.11.5)