FastQCFastQC Report
Mon 6 Jun 2016
ERR1211611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548437
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230690.3056129368238749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201820.26736660847802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145690.19300684366843096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122050.045.580111
TACCTGG65000.040.228572
GTACCTG70150.037.634961
GTATCAA197350.036.072061
ACCTGGG76150.033.384633
TCAACGC250600.028.0332584
ATCAACG249900.027.999333
CAACGCA252950.027.7913975
GTACATG240250.027.236871
TATCAAC262200.026.8511142
AACGCAG266550.026.5155036
TATAACG5450.025.0250342
TACATGG257500.024.875572
ACATGGG264750.023.517933
CGCAGAG303600.022.8617488
ACGCAGA326550.021.528447
CCTGGGG61200.021.4990884
ATGGGGG77550.021.2079985
ATAACGC6800.020.7315393
GCAGAGT340050.019.9413369
Produced by FastQC (version 0.11.5)