FastQCFastQC Report
Mon 6 Jun 2016
ERR1211610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9069258
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280430.309209419337282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242210.26706705223293903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155800.1717891364431357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133450.050.5206341
GTATCAA226500.037.113721
TCAACGC266200.030.821094
CAACGCA269050.030.4569915
ATCAACG270300.030.4231243
AACGCAG278850.029.4841216
TATCAAC289000.028.7280962
GTACATG274750.028.7084371
TACATGG282400.027.184132
ACATGGG286850.025.522573
GTACCTG77200.025.1001221
ACGCAGA325900.025.040257
TACCTGG74850.024.3837152
CATGGGG183650.024.17984
CGCAGAG337100.023.9302228
GCAGAGT370850.020.789579
GAGTACT237250.020.3295912-13
CAGAGTA362300.019.21962710-11
GAGTACA200850.018.7788721
AGAGTAC336800.018.61010710-11
Produced by FastQC (version 0.11.5)