FastQCFastQC Report
Mon 6 Jun 2016
ERR1211610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9069258
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294830.32508723425885555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269250.2968820602523382No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176180.1942606550613071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144900.046.1249581
GTATCAA237000.035.538931
TCAACGC287300.028.5741754
CAACGCA290000.028.2757345
ATCAACG291200.028.175663
AACGCAG302300.027.265456
TATCAAC307250.026.92212
GTACATG294050.026.7684631
TACATGG299650.025.6903152
GTACCTG78900.024.7315581
ACATGGG301550.024.6217543
TACCTGG78650.024.0360092
CATGGGG188700.024.0314
ACGCAGA353300.023.1300837
CGCAGAG364050.022.3438118
GAGTACT247900.019.7048912-13
GCAGAGT408900.019.6860739
ATGGGGG109950.019.1469335
AGAGTAC368000.018.7139410-11
ACCTGGG102150.018.1250463
Produced by FastQC (version 0.11.5)