FastQCFastQC Report
Mon 6 Jun 2016
ERR1211609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398295
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157880.658301001336366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132160.5510581475589951No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121810.5079024890599363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95780.39936705034201386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66470.2771552290272881No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61970.2583918992450887No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27820.11599907434239741No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26270.10953614963963984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24320.10140537340068674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG50900.066.937832
GTACCTG53150.064.8059541
ACCTGGG53700.060.4647143
TAACGCA5650.057.385064
TATAACG5700.054.490392
GTACCCG5500.049.7577361
ATAACGC7050.045.3229333
CCTGGGG34300.044.1123244
TACCCGG6200.040.987642
GTATAAC9400.040.1566471
TATCACG3200.038.2361562
CTGGGGG22100.032.315015
CCTGGGA40250.030.4700264
TGGGATG28700.028.481116
ATCACGC4600.027.5806273
TCACGCA5050.026.983914
CATGGGG63150.026.7127934
ATGGGGG38350.026.3406245
GGTATCA138200.024.684571
ATAGCCC10500.024.1633645