FastQCFastQC Report
Mon 6 Jun 2016
ERR1211609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398295
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175830.7331458390231393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148660.6198570234270596No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126600.5278750112058775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111460.4647468305608776No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA86620.3611732501631367No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.26631419404201734No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37330.15565224461544555No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34290.1429765729403597No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.11487327455546545No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24100.10048805505577922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG45200.066.6974642
TATAACG6300.064.948392
TAACGCA6550.063.8482324
GTACCTG48950.062.1342471
ACCTGGG48950.059.904473
GTACCCG5200.056.2227821
ATAACGC8900.046.4643673
GTATAAC10200.045.3053931
CCTGGGG30700.044.387414
TACCCGG7000.038.9690322
TATCACG2750.037.6252752
CTGGGGG18600.029.8104975
CCTGGGA38350.029.0391034
TACCGTA2600.028.9177787
ACCCGGG9250.028.4494343
CATGGGG63700.027.6625374
GTACCGT3100.027.2853226
ATGGGGG36650.027.180795
ACCGTAT2800.026.8522228
ACAACGC3350.026.652393