##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211609_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2398295
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.970137952170187	33.0	31.0	34.0	30.0	34.0
2	32.202336243039326	33.0	31.0	34.0	30.0	34.0
3	32.28376158896216	34.0	31.0	34.0	30.0	34.0
4	35.95844297719839	37.0	35.0	37.0	35.0	37.0
5	35.8720445149575	37.0	35.0	37.0	35.0	37.0
6	35.91563756752193	37.0	35.0	37.0	35.0	37.0
7	35.8335638443144	37.0	35.0	37.0	35.0	37.0
8	35.81069176227278	37.0	35.0	37.0	35.0	37.0
9	37.41936792596407	39.0	37.0	39.0	34.0	39.0
10-11	37.371691555876154	39.0	37.0	39.0	34.0	39.0
12-13	37.27504018479795	39.0	37.0	39.0	34.0	39.0
14-15	38.41110935060115	40.0	38.0	41.0	33.5	41.0
16-17	38.34485207199281	40.0	38.0	41.0	33.0	41.0
18-19	38.38638053283687	40.0	38.0	41.0	33.5	41.0
20-21	38.323267362855695	40.0	38.0	41.0	34.0	41.0
22-23	38.172704567202956	40.0	37.0	41.0	33.5	41.0
24-25	38.0616225276707	40.0	37.0	41.0	33.0	41.0
26-27	37.836116282609105	40.0	37.0	41.0	33.0	41.0
28-29	37.814235529824316	40.0	37.0	41.0	33.0	41.0
30-31	37.61188823726856	39.5	36.5	41.0	32.5	41.0
32-33	37.289235477703954	39.0	36.0	41.0	32.0	41.0
34-35	37.06593600870619	39.0	35.5	41.0	31.0	41.0
36-37	36.90026643928291	39.0	35.0	41.0	31.0	41.0
38-39	36.7869809593899	39.0	35.0	41.0	30.0	41.0
40-41	36.60928201076181	39.0	35.0	41.0	30.0	41.0
42-43	36.40340804613277	39.0	35.0	41.0	30.0	41.0
44-45	36.15395791593611	39.0	35.0	41.0	28.5	41.0
46-47	35.818348451712566	39.0	35.0	40.0	26.5	41.0
48-49	35.498761620234376	38.5	35.0	40.0	25.5	41.0
50-51	35.269595274976595	38.0	34.0	40.0	24.0	41.0
52-53	35.07710331714823	38.0	34.0	40.0	24.0	41.0
54-55	34.86130897158189	38.0	34.0	40.0	23.5	41.0
56-57	34.64701756873112	38.0	33.0	40.0	23.0	41.0
58-59	34.38397132129283	38.0	33.0	40.0	22.5	41.0
60-61	34.05327138654753	37.0	33.0	40.0	22.0	41.0
62-63	33.66667007186355	36.5	32.0	40.0	21.5	41.0
64-65	33.37269956364834	36.0	31.5	39.0	20.5	41.0
66-67	33.01924784065346	36.0	31.0	39.0	20.0	40.0
68-69	32.6242670313702	35.0	31.0	39.0	19.0	40.0
70-71	32.25138358709	35.0	31.0	38.0	19.0	40.0
72-73	31.833573851423616	35.0	30.0	37.5	18.0	39.5
74-75	31.42583647966576	34.5	29.5	37.0	17.5	39.0
76-77	29.946968575592244	33.0	28.0	35.0	15.5	37.5
78-79	30.80831486535226	34.0	29.0	36.0	18.0	38.0
80-81	30.770781117418835	34.0	29.0	35.5	17.0	37.5
82-83	30.494819653128577	34.0	29.0	35.0	16.0	37.0
84-85	30.167106840484593	34.0	29.0	35.0	15.0	36.5
86-87	29.866250815683642	34.0	29.0	35.0	13.0	36.0
88-89	29.585819926239267	34.0	28.5	35.0	11.5	36.0
90-91	29.288445124557235	34.0	27.0	35.0	10.5	35.0
92-93	29.026962279452697	33.0	27.0	35.0	10.0	35.0
94-95	28.821338909516967	33.0	26.5	35.0	10.0	35.0
96-97	28.585132145962028	33.0	26.0	35.0	10.0	35.0
98-99	28.35546961487223	33.0	25.0	35.0	10.0	35.0
100	26.69559833131454	31.0	23.0	34.0	7.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.24876060910769127
1101	2	0.19625425408786157
1101	3	0.18663243035666
1101	4	0.08617774476387297
1101	5	0.10548528327992557
1101	6	0.14562460596499704
1101	7	0.0829977919406204
1101	8	0.0650692227387708
1101	9	0.0738712194521085
1101	10-11	0.019460053148932843
1101	12-13	0.09090748782493563
1101	14-15	0.06594887443336717
1101	16-17	0.041988935036890496
1101	18-19	0.09763276014141553
1101	20-21	-0.03467182954587855
1101	22-23	0.00936935064818556
1101	24-25	-0.07962364850227743
1101	26-27	-0.061971041037871544
1101	28-29	0.07244907714815696
1101	30-31	0.14393072574033994
1101	32-33	0.07480091319223447
1101	34-35	0.16910180715601797
1101	36-37	0.033406954874145356
1101	38-39	-0.07003476954677268
1101	40-41	-0.10755866580192475
1101	42-43	-0.08444093501077532
1101	44-45	-0.13627141980961
1101	46-47	-0.07687938304969322
1101	48-49	0.01907411935383152
1101	50-51	0.059752025380717555
1101	52-53	-0.14879337687474248
1101	54-55	-0.2926524907747492
1101	56-57	-0.45580951893473554
1101	58-59	-0.3535956276440615
1101	60-61	-0.34253300146730936
1101	62-63	-0.5241044201235141
1101	64-65	-0.29294429816857104
1101	66-67	-0.4026184289781298
1101	68-69	-0.30468678419449446
1101	70-71	-0.5999699682621191
1101	72-73	-0.5512539160293386
1101	74-75	-0.6690144462370924
1101	76-77	-0.5993429087358848
1101	78-79	-0.4686308790327658
1101	80-81	-0.47446501310420075
1101	82-83	-0.49153781171994027
1101	84-85	-0.7630363040754808
1101	86-87	-0.8279264552947083
1101	88-89	-0.7627980450744651
1101	90-91	-0.7235389347528383
1101	92-93	-0.8059056973591403
1101	94-95	-1.0846400020119233
1101	96-97	-1.0360760814967733
1101	98-99	-1.109525175940007
1101	100	-0.8582866520594798
1102	1	0.280894096335345
1102	2	0.18891779406222753
1102	3	0.15656661682389483
1102	4	0.1616733625313671
1102	5	0.15655787927393305
1102	6	0.09221953835585595
1102	7	0.15223933281529156
1102	8	0.06385444064582657
1102	9	0.1714340228555713
1102	10-11	0.1678827488575223
1102	12-13	0.19136956884928935
1102	14-15	0.18766747870257205
1102	16-17	0.12749512568343135
1102	18-19	0.028439938622661032
1102	20-21	0.03781746647413797
1102	22-23	0.08161340571853515
1102	24-25	0.12483097941252197
1102	26-27	0.13797596476397445
1102	28-29	0.21864703765034932
1102	30-31	0.16019875038765719
1102	32-33	0.23436831300299588
1102	34-35	0.15597354163951138
1102	36-37	0.10244063803494896
1102	38-39	0.20190681014761935
1102	40-41	0.02165224851068359
1102	42-43	-0.07492646882347742
1102	44-45	0.16243283570065614
1102	46-47	0.05447128883609054
1102	48-49	0.022169572427394257
1102	50-51	0.031296806256975174
1102	52-53	-0.007532339950323319
1102	54-55	-0.029185915627785164
1102	56-57	-0.006960429346328567
1102	58-59	-0.04098342385212561
1102	60-61	-0.18175324255110326
1102	62-63	-0.28579503196420575
1102	64-65	-0.0952843329661377
1102	66-67	-0.24669182750021434
1102	68-69	-0.4881309086315184
1102	70-71	-0.452423874818912
1102	72-73	-0.3403429783002885
1102	74-75	-0.27765644654481036
1102	76-77	-0.30494412895239265
1102	78-79	-0.3450099699659255
1102	80-81	-0.21911994593465778
1102	82-83	-0.3121195675586037
1102	84-85	-0.38403891553680225
1102	86-87	-0.34375271739861546
1102	88-89	-0.27990355804601563
1102	90-91	-0.2956616673773773
1102	92-93	-0.4890807836477862
1102	94-95	-0.5095022941131049
1102	96-97	-0.41795120037459554
1102	98-99	-0.6100322726108054
1102	100	-0.6399962462765707
1103	1	0.3105504391224656
1103	2	0.21089810878902426
1103	3	0.19046564751869965
1103	4	0.09888469502438113
1103	5	0.19430414612991598
1103	6	0.0637911773079054
1103	7	0.12325205986373788
1103	8	0.13531275737508963
1103	9	0.2624662703633547
1103	10-11	0.27344366001760534
1103	12-13	0.2813588694851745
1103	14-15	0.21356440708992608
1103	16-17	0.17377761698543992
1103	18-19	0.20826827838356365
1103	20-21	0.25176337605292076
1103	22-23	0.16774430210797675
1103	24-25	0.12549928778771857
1103	26-27	0.10180008298864607
1103	28-29	0.21811943022816394
1103	30-31	0.35151634247402086
1103	32-33	0.41500481602506767
1103	34-35	0.3013223640918312
1103	36-37	0.3125541176262061
1103	38-39	0.3958595627168293
1103	40-41	0.3811852330274874
1103	42-43	0.31456921936492677
1103	44-45	0.31018305030556803
1103	46-47	0.4436186512135478
1103	48-49	0.3849410478516049
1103	50-51	0.20706808522440667
1103	52-53	0.3320151021605042
1103	54-55	0.36364801477508024
1103	56-57	0.14076567234721438
1103	58-59	0.06651464246159833
1103	60-61	0.14294343817551436
1103	62-63	0.34314855008407363
1103	64-65	0.19295277969801816
1103	66-67	0.10355142524184657
1103	68-69	0.07350617699233197
1103	70-71	0.21892458856254393
1103	72-73	-0.014202822552878303
1103	74-75	-0.11638667247686385
1103	76-77	-0.03520703414025306
1103	78-79	-0.1447489413548162
1103	80-81	-0.019854569046472648
1103	82-83	-0.05996856895410829
1103	84-85	-0.07542808489692376
1103	86-87	0.029479277405854987
1103	88-89	0.015311154315718056
1103	90-91	0.05001806882567905
1103	92-93	-0.013772320286633999
1103	94-95	-0.07304819164389187
1103	96-97	-0.0011385448357437156
1103	98-99	0.03491331427799693
1103	100	0.03753409072717062
1104	1	0.38521326686787916
1104	2	0.26760326256485456
1104	3	0.2631246861499079
1104	4	0.18797044198156243
1104	5	0.20900615656569244
1104	6	0.2359006380882036
1104	7	0.22942145295110095
1104	8	0.20859970832322006
1104	9	0.2548859809706556
1104	10-11	0.2730603174871433
1104	12-13	0.30517488293239126
1104	14-15	0.38343675849274206
1104	16-17	0.34626148937702794
1104	18-19	0.370982240450175
1104	20-21	0.352114017679348
1104	22-23	0.29590350742105187
1104	24-25	0.1891332891022799
1104	26-27	0.2531041946979613
1104	28-29	0.26156156791924445
1104	30-31	0.3284324926501725
1104	32-33	0.3236463218879564
1104	34-35	0.3306636967807677
1104	36-37	0.33537287372435287
1104	38-39	0.33960215713853614
1104	40-41	0.4302389935541626
1104	42-43	0.2423053739069303
1104	44-45	0.3333638521269222
1104	46-47	0.30823081145003783
1104	48-49	0.1967906257540335
1104	50-51	0.1881886373475723
1104	52-53	0.14405016838413331
1104	54-55	-0.002965615971604052
1104	56-57	0.1711464266286029
1104	58-59	0.13329979838084682
1104	60-61	0.13115359438243956
1104	62-63	0.07681136563535063
1104	64-65	0.30396522132716797
1104	66-67	0.366214395194973
1104	68-69	0.1473892667949528
1104	70-71	0.27334557388681446
1104	72-73	0.24076796389527644
1104	74-75	0.2188385558583974
1104	76-77	0.17535724709507505
1104	78-79	0.23479571573137648
1104	80-81	0.32596745250486947
1104	82-83	0.16443649013680428
1104	84-85	0.10414006737662262
1104	86-87	0.061061068566960586
1104	88-89	0.06417126847003729
1104	90-91	0.020894513165700346
1104	92-93	-0.020286395329367934
1104	94-95	-0.08885574682366126
1104	96-97	-0.2827105033983379
1104	98-99	-0.09616123502402374
1104	100	-0.09090094258944958
1105	1	0.24792527577493217
1105	2	0.22130329438781615
1105	3	0.11291623305839238
1105	4	0.12596242124345025
1105	5	0.09175211559763596
1105	6	0.06252709132996159
1105	7	0.10817595100500199
1105	8	0.13242109791482903
1105	9	0.12293378126086196
1105	10-11	0.21138867478899215
1105	12-13	0.21154842378184924
1105	14-15	0.21045006758322415
1105	16-17	0.08209187043383537
1105	18-19	0.1635609896303336
1105	20-21	0.15097252703082376
1105	22-23	0.18696142268754556
1105	24-25	0.01303833004585897
1105	26-27	0.10684027003988916
1105	28-29	0.10660946697037588
1105	30-31	0.26246483390041675
1105	32-33	0.20369543223768005
1105	34-35	0.21886185003577907
1105	36-37	0.10379132294139026
1105	38-39	0.08104891191346297
1105	40-41	0.024901260716831075
1105	42-43	0.09057937893276602
1105	44-45	-0.015101850396717964
1105	46-47	-0.05246343568357048
1105	48-49	-0.15462752211555397
1105	50-51	-0.05732487306326561
1105	52-53	-0.10245652245786374
1105	54-55	-0.014245549185581297
1105	56-57	-0.14670304989626715
1105	58-59	-0.08414171953244676
1105	60-61	-0.06444192136486748
1105	62-63	-0.1671892455006585
1105	64-65	-0.0742488771754708
1105	66-67	-0.006824292922232189
1105	68-69	-0.1806261702981331
1105	70-71	-0.14090284277288845
1105	72-73	0.005266110650641309
1105	74-75	-0.0840122585816303
1105	76-77	-0.058846195356423436
1105	78-79	0.0918043231262935
1105	80-81	-0.08375788237487569
1105	82-83	0.08065404780432672
1105	84-85	-0.07792494770341207
1105	86-87	-0.04661451275084971
1105	88-89	-0.09625590209683921
1105	90-91	-0.201395237897664
1105	92-93	-0.26863155887156154
1105	94-95	-0.2260016741170503
1105	96-97	-0.04491275396578942
1105	98-99	-0.005374110410521382
1105	100	0.14091003743129704
1106	1	0.22456360160644095
1106	2	0.14307440702811647
1106	3	0.09491136130401401
1106	4	0.08839476367034393
1106	5	0.10930172296892238
1106	6	0.09774789537399187
1106	7	0.13432501490648718
1106	8	0.09194691600381333
1106	9	0.15371403928219962
1106	10-11	0.15258011902606228
1106	12-13	0.13535497078478897
1106	14-15	0.1764247825725036
1106	16-17	0.17243010306869877
1106	18-19	0.16163470744005082
1106	20-21	0.20452688669460173
1106	22-23	0.1487824638552624
1106	24-25	0.2033714078180111
1106	26-27	0.14236701109476968
1106	28-29	0.17697538858210038
1106	30-31	0.18766695770386121
1106	32-33	0.2661530580388316
1106	34-35	0.3039362314317984
1106	36-37	0.3465865022447048
1106	38-39	0.37844218784508143
1106	40-41	0.24013911671942623
1106	42-43	0.3233711789371796
1106	44-45	0.357069009573479
1106	46-47	0.42346679202226056
1106	48-49	0.5930698498045146
1106	50-51	0.33999607114393626
1106	52-53	0.43637301277808405
1106	54-55	0.3847545710945539
1106	56-57	0.41776693612015237
1106	58-59	0.4052975214099348
1106	60-61	0.42984411787590915
1106	62-63	0.5539283451001396
1106	64-65	0.5426760099289467
1106	66-67	0.4966125276893649
1106	68-69	0.39604954874385356
1106	70-71	0.5162355374710543
1106	72-73	0.28719070728262963
1106	74-75	0.3140572862439903
1106	76-77	0.4795728327964923
1106	78-79	0.3766192430990962
1106	80-81	0.39945608452108416
1106	82-83	0.44588580797719857
1106	84-85	0.49245962960193523
1106	86-87	0.3739347337800538
1106	88-89	0.4098854213282479
1106	90-91	0.3125654395003892
1106	92-93	0.4021330054566228
1106	94-95	0.4773590509021197
1106	96-97	0.5235200941285854
1106	98-99	0.49918196999551867
1106	100	0.6033922101977502
1107	1	0.23382947047485914
1107	2	0.20804065707744002
1107	3	0.16723235808114367
1107	4	0.1139468302943385
1107	5	0.07692792371810953
1107	6	0.13415231823336882
1107	7	0.09060497758734698
1107	8	0.043264744045544035
1107	9	0.22238921308653659
1107	10-11	0.10414252374306443
1107	12-13	0.15397964765823957
1107	14-15	0.13256468434398272
1107	16-17	0.16984843321004206
1107	18-19	0.09525121708306017
1107	20-21	0.09829182829131611
1107	22-23	0.15551117581992457
1107	24-25	0.08913029500065761
1107	26-27	0.15690774664444262
1107	28-29	0.1147349455787392
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2316	74-75	0.12647175956026047
2316	76-77	0.20284708280928143
2316	78-79	-0.1312946525030192
2316	80-81	0.0358611049820432
2316	82-83	0.16758659012244337
2316	84-85	0.32290974687017737
2316	86-87	0.2230539130570719
2316	88-89	0.1657123914763119
2316	90-91	0.16270885659334766
2316	92-93	0.3193731471304595
2316	94-95	0.5171218935889499
2316	96-97	0.391384193864301
2316	98-99	0.39284932147062435
2316	100	0.207277680002516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	11.0
11	21.0
12	44.0
13	156.0
14	540.0
15	1384.0
16	3415.0
17	7514.0
18	13941.0
19	19313.0
20	23174.0
21	27863.0
22	32957.0
23	34283.0
24	33806.0
25	34522.0
26	37564.0
27	43539.0
28	50324.0
29	59873.0
30	70027.0
31	83431.0
32	99534.0
33	123758.0
34	161776.0
35	216220.0
36	296853.0
37	440410.0
38	418555.0
39	63476.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.85476333394084	18.7157967425708	12.635287311542456	21.794152611945915
2	15.190087341656474	21.972742192517185	41.3788556494583	21.458314816368038
3	17.031057672100633	31.328411406993773	30.735019897858773	20.905511023046824
4	10.946603571159033	17.922968607775026	38.76833666281806	32.362091158247885
5	10.827288753829809	37.87824835341151	34.62593378954689	16.668529103211785
6	26.259277482195575	37.00214320262855	22.109803692646395	14.62877562252948
7	25.535349070902452	33.238571568551826	24.534846630627175	16.691232729918546
8	26.97713186827849	33.54019060390148	22.351468057001043	17.13120947081899
9	28.10759310259997	15.763156742602558	22.144064846067728	33.98518530872974
10-11	23.97331343578569	26.691296761790873	29.95569126361311	19.37969853881033
12-13	25.01171162324662	24.964260524876696	29.92834175298451	20.095686098892177
14-15	22.741702488997912	25.462198241291883	29.0902138999193	22.705885369790906
16-17	20.847966145900113	27.70328377513743	29.942440274670528	21.50630980429193
18-19	20.637147440808835	26.281388544021883	32.67977821489888	20.401685800270403
20-21	21.411551659399944	26.15115157375457	32.65094319364256	19.786353573202923
22-23	20.955724995408197	26.14578818406163	32.14079838818794	20.757688432342235
24-25	20.431164729769428	26.33575651363917	32.38384610397097	20.849232652620437
26-27	20.563443119889588	26.016161515079496	32.41436606915761	21.00602929587331
28-29	20.67404397195295	26.162990940083127	32.651893709428855	20.51107137853507
30-31	21.541493241310526	26.294842147135867	32.58427200545732	19.579392606096288
32-33	20.805656533309318	26.36486758084545	32.746334987061225	20.083140898784013
34-35	20.88768937439342	26.798098984964717	32.24570171076759	20.06850992987427
36-37	21.311374585140985	26.667082246692335	31.847516600093943	20.174026568072737
38-39	21.487414459585143	27.050838156899204	31.392099560984793	20.069647822530857
40-41	21.234124398621848	26.92276818989737	31.760564187833268	20.082543223647516
42-43	21.08689709700806	26.88764743327906	31.289122584462874	20.73633288525001
44-45	20.957000887370647	27.181793389422275	31.332878250078394	20.528327473128684
46-47	20.84657710696146	26.842266682150896	31.188823669348675	21.12233254153897
48-49	20.812356204111715	27.0991463202326	31.2207651948014	20.867732280854284
50-51	20.76878748176732	27.524992014731257	30.94220750356731	20.764012999934117
52-53	20.775542702884593	27.81507999005089	29.959892911551123	21.449484395513394
54-55	20.705967271110122	27.730154391508645	30.380044004634392	21.18383433274684
56-57	21.0967260257322	27.673779705333352	29.838214178416617	21.39128009051783
58-59	21.01478705070829	27.768324113404496	30.35269094467751	20.864197891209706
60-61	21.70245256468556	28.14293425807255	30.020414235296006	20.134198941945886
62-63	21.08110136810928	27.968040266595217	30.514727269504917	20.436131095790586
64-65	20.88156144728231	27.546084758049936	30.23874858039159	21.33360521427617
66-67	21.03660246523972	27.60320811841524	30.056167300535613	21.304022115809428
68-69	21.027709901046045	27.721264997548932	30.092348265704615	21.15867683570041
70-71	20.860481519218425	27.76208880023835	30.171811504596924	21.205618175946306
72-73	20.8329006798602	28.176500098207125	29.46305514653296	21.52754407539972
74-75	21.032718938879487	28.099523519910395	29.46284912700916	21.404908414200953
76-77	20.70761710308524	27.758227035510952	30.292903917999332	21.241251943404478
78-79	20.661246806854052	27.81597887660064	30.075155046510304	21.447619270035005
80-81	20.518968484720858	28.27891402545116	29.496783421197208	21.705334068630773
82-83	20.888904735900173	28.511275461299203	29.217968759285355	21.38185104351527
84-85	20.586448059262892	28.755948873160076	28.88717754302728	21.77042552454975
86-87	20.4432453419225	28.33498998813339	29.48205401841483	21.739710651529283
88-89	20.528551321482563	28.166230516603243	29.5804201636866	21.724797998227597
90-91	21.260046138516486	28.413205408992383	29.297290623854305	21.029457828636826
92-93	20.545371966419527	28.573513900595692	29.426204997032578	21.454909135952203
94-95	20.255607951023908	28.314907794449233	29.310996347146993	22.118487907379862
96-97	20.596899897241535	28.3324770127981	29.323466463373947	21.747156626586417
98-99	20.756369790331927	27.95063434647914	29.220515863822826	22.072479999366102
100	19.376280058836308	29.045986823969415	29.49263899703024	22.08509412016403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1708.0
1	1486.0
2	4974.0
3	7512.0
4	4377.5
5	2545.5
6	3270.5
7	5658.5
8	8421.5
9	9343.5
10	7765.0
11	5989.0
12	5908.5
13	6248.0
14	6604.5
15	6886.5
16	7244.0
17	7684.5
18	7917.0
19	8092.5
20	8429.5
21	8909.5
22	9662.0
23	10900.0
24	12303.0
25	14250.0
26	17040.5
27	19925.0
28	22954.0
29	27088.5
30	32278.5
31	37183.0
32	42820.0
33	49418.0
34	56587.5
35	62728.5
36	70939.5
37	79042.5
38	81690.0
39	83191.0
40	80644.0
41	76810.5
42	74740.0
43	73304.0
44	74341.5
45	77110.0
46	77997.0
47	76356.0
48	76892.5
49	80968.0
50	92383.5
51	92086.0
52	77246.0
53	68321.0
54	63804.5
55	59148.5
56	53531.0
57	49359.5
58	44770.0
59	37887.0
60	29764.5
61	22726.0
62	17937.5
63	13858.0
64	10245.0
65	7610.5
66	5630.5
67	4200.5
68	3235.5
69	2444.0
70	1833.0
71	1336.5
72	987.5
73	749.5
74	593.0
75	440.0
76	285.5
77	183.5
78	126.0
79	87.0
80	59.0
81	36.5
82	27.0
83	20.0
84	14.5
85	9.5
86	8.5
87	8.0
88	5.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2493021083728232
2	0.07688795581861281
3	0.001959725555029719
4	0.0010841034985270787
5	1.2508886521466291E-4
6	6.254443260733146E-4
7	0.0
8	9.590146333124158E-4
9	0.0
10-11	3.961147398464326E-4
12-13	0.001521914526778399
14-15	2.0848144202443817E-5
16-17	1.4593700941710672E-4
18-19	0.00114664793113441
20-21	0.0013968256615637358
22-23	2.0848144202443817E-5
24-25	2.0848144202443817E-5
26-27	2.0848144202443818E-4
28-29	0.0016261552477906179
30-31	0.0020431181318394946
32-33	0.0032106142071763478
34-35	0.004273869561500983
36-37	0.0016886996803979495
38-39	0.003606728947022781
40-41	0.006567165423769804
42-43	0.012654823530883397
44-45	0.00829756139257264
46-47	0.006483772846960028
48-49	0.006150202539720927
50-51	0.0053788212042305055
52-53	0.0037735141006423312
54-55	0.005774935944076937
56-57	0.003898602965856994
58-59	0.0020014218434346066
60-61	0.008902157574443511
62-63	0.011570720032356319
64-65	0.007776357787511544
66-67	0.007984839229535983
68-69	0.015073208258366881
70-71	0.004774225022359634
72-73	0.012592279098276067
74-75	0.00785975036432132
76-77	0.004982706464384073
78-79	0.016073919180084186
80-81	0.007192609749843118
82-83	0.015281689700391319
84-85	0.02385027696759573
86-87	0.016282400622108624
88-89	0.01751244113005281
90-91	0.030521683112377754
92-93	0.02468420273569348
94-95	0.029896238786304437
96-97	0.018304670609745673
98-99	0.018075341023518793
100	0.019013507512628765
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2398295.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.17017116686528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65863932668415	46.542451527126225
2	14.09025166508722	16.674452056148386
3	3.5585217723324023	6.31675027109775
4	1.2766626873752887	3.0216139893738423
5	0.630124867910948	1.8642298145394582
6	0.38625254748541565	1.3712777609009883
7	0.25756369518063194	1.0668061551145815
8	0.18042575300199473	0.8540658150430867
9	0.13657383471609427	0.7272987459359819
>10	0.7155846102505391	7.656809419790578
>50	0.059739837332657156	2.458760260508085
>100	0.043876065951294466	4.954311021416947
>500	0.003935881915011467	1.6498842049886189
>1k	0.001365510052146828	1.4523581706528206
>5k	1.606482414290386E-4	0.7152476637919463
>10k+	3.212964828580772E-4	2.6736831235707315
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17583	0.7331458390231393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14866	0.6198570234270596	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12660	0.5278750112058775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11146	0.4647468305608776	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8662	0.3611732501631367	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6387	0.26631419404201734	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	3733	0.15565224461544555	No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	3429	0.1429765729403597	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2755	0.11487327455546545	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2410	0.10048805505577922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	4.169628840488764E-5
9	0.0	0.0	0.0	0.0	4.169628840488764E-5
10-11	0.0	0.0	0.0	4.169628840488764E-5	4.169628840488764E-5
12-13	0.0	0.0	0.0	4.169628840488764E-5	4.169628840488764E-5
14-15	0.0	0.0	0.0	4.169628840488764E-5	4.169628840488764E-5
16-17	0.0	0.0	0.0	4.169628840488764E-5	4.169628840488764E-5
18-19	0.0	0.0	0.0	4.169628840488764E-5	8.339257680977528E-5
20-21	0.0	0.0	0.0	6.254443260733146E-5	8.339257680977528E-5
22-23	0.0	0.0	0.0	2.9187401883421345E-4	1.042407210122191E-4
24-25	0.0	0.0	0.0	6.254443260733146E-4	1.2508886521466291E-4
26-27	0.0	0.0	0.0	0.0012091923637417416	1.2508886521466291E-4
28-29	0.0	0.0	0.0	0.0033357030723910113	1.2508886521466291E-4
30-31	0.0	0.0	0.0	0.008193320671560422	1.2508886521466291E-4
32-33	0.0	0.0	0.0	0.019034355656831207	1.2508886521466291E-4
34-35	0.0	0.0	0.0	0.032314623513787916	1.2508886521466291E-4
36-37	0.0	0.0	0.0	0.052516475245955985	1.2508886521466291E-4
38-39	0.0	0.0	0.0	0.08945938677268643	1.2508886521466291E-4
40-41	0.0	0.0	0.0	0.1425387619121084	1.2508886521466291E-4
42-43	0.0	0.0	0.0	0.18692446091911127	1.2508886521466291E-4
44-45	2.084814420244382E-5	0.0	0.0	0.2339370260956221	1.2508886521466291E-4
46-47	4.169628840488764E-5	0.0	0.0	0.28539024598725343	1.6678515361955056E-4
48-49	4.169628840488764E-5	0.0	0.0	0.34522441984826724	1.6678515361955056E-4
50-51	4.169628840488764E-5	0.0	0.0	0.4112296443932043	1.6678515361955056E-4
52-53	4.169628840488764E-5	0.0	0.0	0.47938222779099315	2.084814420244382E-4
54-55	4.169628840488764E-5	0.0	0.0	0.5582299091646357	2.5017773042932583E-4
56-57	4.169628840488764E-5	0.0	0.0	0.6388705309396885	2.5017773042932583E-4
58-59	4.169628840488764E-5	0.0	0.0	0.7313112023333244	2.5017773042932583E-4
60-61	4.169628840488764E-5	0.0	0.0	0.8221048703349672	2.5017773042932583E-4
62-63	4.169628840488764E-5	0.0	0.0	0.9204664146820971	2.5017773042932583E-4
64-65	4.169628840488764E-5	0.0	0.0	1.0188071108850245	2.5017773042932583E-4
66-67	4.169628840488764E-5	0.0	0.0	1.12532027961531	2.5017773042932583E-4
68-69	4.169628840488764E-5	0.0	0.0	1.2509511965792366	2.5017773042932583E-4
70-71	4.169628840488764E-5	0.0	0.0	1.3917178662341372	2.5017773042932583E-4
72-73	4.169628840488764E-5	0.0	0.0	1.5163897685647512	2.5017773042932583E-4
74-75	4.169628840488764E-5	0.0	0.0	1.641749659654046	2.5017773042932583E-4
76-77	4.169628840488764E-5	0.0	0.0	1.775115238117079	2.5017773042932583E-4
78-79	4.169628840488764E-5	0.0	0.0	1.9314554714912053	2.5017773042932583E-4
80-81	4.169628840488764E-5	0.0	0.0	2.078768458425673	2.5017773042932583E-4
82-83	4.169628840488764E-5	0.0	0.0	2.2381733689975585	2.5017773042932583E-4
84-85	4.169628840488764E-5	0.0	0.0	2.401560275112111	2.5017773042932583E-4
86-87	4.169628840488764E-5	0.0	0.0	2.5665107920418464	2.5017773042932583E-4
88	4.169628840488764E-5	0.0	0.0	2.6964572748556788	2.5017773042932583E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	4520	0.0	66.697464	2
TATAACG	630	0.0	64.94839	2
TAACGCA	655	0.0	63.848232	4
GTACCTG	4895	0.0	62.134247	1
ACCTGGG	4895	0.0	59.90447	3
GTACCCG	520	0.0	56.222782	1
ATAACGC	890	0.0	46.464367	3
GTATAAC	1020	0.0	45.305393	1
CCTGGGG	3070	0.0	44.38741	4
TACCCGG	700	0.0	38.969032	2
TATCACG	275	0.0	37.625275	2
CTGGGGG	1860	0.0	29.810497	5
CCTGGGA	3835	0.0	29.039103	4
TACCGTA	260	0.0	28.917778	7
ACCCGGG	925	0.0	28.449434	3
CATGGGG	6370	0.0	27.662537	4
GTACCGT	310	0.0	27.285322	6
ATGGGGG	3665	0.0	27.18079	5
ACCGTAT	280	0.0	26.852222	8
ACAACGC	335	0.0	26.65239	3
>>END_MODULE