FastQCFastQC Report
Mon 6 Jun 2016
ERR1211608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996582
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184640.36953261249390085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159920.32005879219034133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109290.21872952350226615No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90470.18106377519672448No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56060.1121966976625221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG65150.049.5095441
TACCTGG64950.048.8867572
GGTATCA84650.047.3520931
ACCTGGG77800.040.105793
GTATCAA139300.036.324181
TATAACG6250.030.8581892
TCAACGC170200.028.934794
TAACGCA7150.028.9177974
ATCAACG172400.028.6200663
CAACGCA173100.028.4477565
CCTGGGG74050.027.8585194
GTACATG153750.027.6348361
TATCAAC181250.027.5621512
AACGCAG180500.027.4598526
TACATGG157600.026.4450322
ACATGGG161150.025.0776423
ACGCAGA203900.024.055777
CATGGGG101100.023.9372734
ATAACGC8000.023.495713
CGCAGAG210850.023.1744148
Produced by FastQC (version 0.11.5)